Anti-FCN1 Rabbit Polyclonal Antibody for WB and ELISA - O00602

Core Product Specifications and Parameters

Biological Background: Ficolin-1 Function and Localization

• Ficolin-1 (also known as M-ficolin, ficolin-A, ficolin-alpha) is a 35 kDa secreted collagen-like lectin encoded by the FCN1 gene (GeneID: 2219) belonging to the ficolin family of pattern recognition molecules.
• Acts as a pattern recognition receptor that binds specific carbohydrate structures on pathogen surfaces to initiate the lectin complement pathway via MASP1 serine protease activation, leading to pathogen phagocytosis and adaptive immune signaling. Related references: PMID:16116205, PMID:17897951, PMID:20032467
• Preferentially recognizes 9-O-acetylated 2-6-linked sialic acid derivatives and glycans containing sialic acid with 2-3 linkages. Related references: PMID:20032467, PMID:20400674
• May also activate monocytes through the G protein-coupled receptor FFAR2, inducing interleukin-8/IL-8 secretion. Related references: PMID:21037097
• Localized as a secreted protein and tethered on the cell surface of immune cells.
• Expressed in peripheral blood leukocytes, monocytes, and granulocytes; also detected in spleen, lung, and thymus (attributed to tissue macrophages). Not found on lymphocytes.
• Contains a collagen-like region and a fibrinogen-like lectin domain; calcium-dependent binding and disulfide bond formation contribute to its oligomerization and structural integrity (3D structure solved; proteomics identification; reference proteome; signal peptide).

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 30–326, encompassing nearly full-length FCN1; the resulting polyclonal antibody is expected to target multiple epitopes, providing robust signal in both denaturing (WB) and native (ELISA) conditions.
• For Western blot, reduce samples with DTT/β-mercaptoethanol to detect the 35 kDa monomeric form; note that disulfide-linked oligomers may appear at higher molecular masses under non-reducing conditions.
• Validate specificity in known FCN1-expressing samples (e.g., human peripheral blood mononuclear cells, U937 or THP-1 monocytic lines) and use a negative control (lymphocytes or knockdown) to confirm the band of interest.
• In ELISA, conduct checkerboard titrations using recombinant ficolin-1 or serum to optimize coating and detection antibody concentrations; consider testing the antibody as both capture and detection components.
• When using with mouse or rat samples, verify reactivity with species-specific positive controls, as sequence homology is ~80% but epitope recognition may vary.
• Collect blood and prepare serum/plasma under conditions that prevent unintended complement activation to avoid protein consumption or complex formation.
• Lot-to-lot consistency may differ among polyclonal antibodies; request retention samples or perform a bridging study if switching between lots for critical assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials and technical support, streamlining your journey from concept to clinic. This anti-FCN1 (ficolin-1) antibody supports innate immunity research and complement pathway assay development. We supply validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and can accommodate rare target sourcing—all designed to meet procurement needs for diagnostic manufacturers and research laboratories.