Anti-FCHO2 Polyclonal Antibody for WB and ELISA - Q0JRZ9

Core Product Specifications and Parameters

Biological Background: FCHO2 Function and Localization

• FCHO2, or F-BAR domain only protein 2, is a key player in the early steps of clathrin-mediated endocytosis, acting as a membrane-binding and bending factor that facilitates the formation of clathrin-coated vesicles.
• It preferentially binds to membranes enriched in phosphatidylserine and phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), common lipid components of the plasma membrane, thereby linking membrane curvature with cargo recruitment.
• FCHO2 recruits adaptor proteins, such as the AP-2 complex, and other endocytic factors to nascent clathrin-coated pits, a critical step for the assembly of functional endocytic machinery.
• The protein mediates both AP-2-dependent endocytosis, exemplified by the uptake of the transferrin receptor, and AP-2-independent pathways, such as the internalization of the low-density lipoprotein (LDL) receptor.
• Subcellularly, FCHO2 localizes to the plasma membrane and clathrin-coated pits, consistent with its role in vesicle formation.
• Post-translational modifications, including phosphorylation and ubiquitination (isopeptide bond formation), regulate FCHO2 function, while alternative splicing generates multiple isoforms (UniProt keywords: Phosphoprotein, Ubl conjugation, Alternative splicing).
• Its predicted molecular weight is approximately 89 kDa, and it has been identified through proteomics approaches (Reference proteome, Proteomics identification).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-300) of human FCHO2, which may enable recognition of the full-length protein as well as potential isoforms arising from alternative splicing. Researchers should validate isoform specificity in their experimental context.
• For Western blot (WB), the calculated molecular weight of FCHO2 is 89 kDa. It is advisable to include appropriate positive controls (e.g., human or mouse cell lysates) and optimize antibody dilution for signal specificity.
• ELISA applications can be used for detecting FCHO2 in samples; consider pairing with a detection antibody and validating against known standards.
• Cross-reactivity with human and mouse FCHO2 has been noted, but due to the polyclonal nature of the antibody, performance should be verified in the relevant species and tissue types.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and institutes with comprehensive access to IVD raw materials, technical services, and consulting, covering the entire development pipeline from concept to clinic. This anti-FCHO2 polyclonal antibody serves as a tool for investigating clathrin-mediated endocytosis and membrane trafficking, areas relevant to numerous diagnostic targets. Beyond this product, CamelBio offers a one-stop supply of validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare target raw materials to support your assay development and research needs.