Anti-FCGR3B Rabbit Polyclonal Antibody for WB, ELISA - O75015

Core Product Specifications and Parameters

Biological Background: FCGR3B Function and Localization

• Encoded by the FCGR3B gene, also known as CD16b, Fc-gamma RIII-beta, or FcRIIIb, FCGR3B is a low-affinity receptor for the Fc region of immunoglobulin gamma (IgG).
• Unlike the highly related FCGR3A (CD16a), FCGR3B is anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) anchor and lacks a transmembrane domain, consistent with its inability to mediate antibody-dependent cellular cytotoxicity (ADCC) or phagocytosis.
• FCGR3B binds both complexed/aggregated IgG and monomeric IgG, and is thought to function primarily as a trap for immune complexes in the peripheral circulation, preventing aberrant neutrophil activation.
• The protein is specifically expressed on the surface of polymorphonuclear leukocytes (neutrophils) and, to a lesser extent, on stimulated eosinophils, with a secreted form also detected.
• Mature FCGR3B contains two extracellular immunoglobulin-like domains stabilized by disulfide bonds and is heavily glycosylated, contributing to a molecular weight of approximately 26 kDa.
• As a member of the immunoglobulin superfamily, this receptor plays a key role in immune complex clearance and the regulation of inflammatory responses.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 1-233 of human FCGR3B) encompasses the entire extracellular region, which is expected to generate antibodies recognizing native and denatured forms.
• For Western blotting, anticipate a band near 26 kDa under reducing conditions; however, extensive glycosylation and potential dimerization may lead to higher apparent molecular weights; validate using neutrophil or transfected cell lysates.
• In ELISA applications, consider using the antibody for either capture or detection of recombinant or native FCGR3B; cross-reactivity with rat FCGR3B expands utility in rodent models, but individual experimental titration is recommended.
• As a polyclonal antibody, lot-to-lot consistency should be verified in the intended assay; include appropriate controls (e.g., antigen blocking, isotype control) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with integrated access to high-quality IVD raw materials, including validated antibody pairs, optimized recombinant proteins, and ancillary reagents. Supporting immune receptor research, this FCGR3B antibody complements our portfolio of antibodies targeting Fc receptors, immune checkpoint molecules, and inflammation-related biomarkers. We offer tailored sourcing for rare targets, bulk supply, and technical consultation to accelerate your assay development from concept to clinic.