Anti-FCGR3B Rabbit Polyclonal Antibody for WB and ELISA - O75015

Core Product Specifications and Parameters

Biological Background: FCGR3B Function and Localization

• FCGR3B (Fc gamma receptor III-B, CD16b) is a low-affinity receptor for the Fc region of immunoglobulin gamma (IgG), belonging to the immunoglobulin superfamily.
• Unlike the closely related FCGR3A (CD16a), FCGR3B lacks a transmembrane domain and is anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) anchor; it is also found in a secreted form.
• This receptor binds both complexed/aggregated IgG and monomeric IgG but does not mediate antibody-dependent cellular cytotoxicity (ADCC) or phagocytosis, potentially acting as a circulating trap for immune complexes on neutrophils.
• Encoded by the FCGR3B gene, the protein is expressed specifically by polymorphonuclear leukocytes (neutrophils) and to a lesser extent by stimulated eosinophils.
• Key post-translational modifications include N-linked glycosylation and disulfide bond formation, and the protein contains immunoglobulin-like domains.
• The molecular weight of the mature protein is approximately 26 kDa, consistent with its glycoprotein nature.

Experimental Guidance and Technical Tips

• The immunogen comprises the entire extracellular domain (amino acids 1-233) of human FCGR3B, making this antibody suitable for detecting both membrane-bound and secreted forms of the receptor.
• For Western blot (WB), a band is expected around 26 kDa under reducing conditions; appropriate loading controls and validation in neutrophil or eosinophil lysates are recommended.
• In ELISA applications, the antibody may be used as a capture or detection reagent for FCGR3B; consider optimizing coating concentrations and validating in relevant sample matrices such as serum or cell culture supernatants.
• Since cross-reactivity with mouse and rat is reported, this antibody can be utilized in translational studies; however, sequence alignment and empirical testing in these species are advised.

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