Anti-FCGR3A Polyclonal Antibody for WB, ELISA - P08637

Core Product Specifications and Parameters

Biological Background: FCGR3A Function and Localization

• FCGR3A (CD16a) is a low-affinity transmembrane receptor for the Fc region of immunoglobulin G (IgG), belonging to the immunoglobulin superfamily. It is also known as Fc-gamma RIII-alpha and is a glycoprotein with immunoglobulin-like domains and disulfide bonds.
• Functions as a receptor for clustered antigen–IgG immune complexes, triggering antibody-dependent cellular cytotoxicity (ADCC). It does not bind free monomeric IgG, thereby preventing inappropriate effector cell activation. Related references: PMID:11711607 PMID:21768335 PMID:22023369
• Expressed on natural killer (NK) cells, mediates IgG effector functions including cytokine production and degranulation in response to infection, contributing to the generation of memory-like adaptive NK cells and regulating NK cell survival and proliferation. Related references: PMID:24412922 PMID:25786175 PMID:29967280 PMID:9916693
• Engagement of antigen–IgG complexes induces phosphorylation of ITAM-containing adapters, activating phosphatidylinositol 3-kinase signaling and elevating intracellular calcium, which drives NK cell activation and degranulation. Related references: PMID:1825220 PMID:23024279 PMID:2532305
• Plays a key role in the antitumor activity of therapeutic antibodies, mediating enhanced ADCC, especially in response to afucosylated IgGs. It can also costimulate NK cells independently of IgG binding, and on monocytes triggers TNFA-dependent ADCC of tumor cells. Related references: PMID:10318937 PMID:23006327 PMID:27670158 PMID:34485821 PMID:28566370
• (Microbial infection) Involved in Dengue virus pathogenesis by mediating antibody-dependent enhancement (ADE), facilitating viral entry into myeloid cells through high-affinity binding of virus–IgG complexes.
• Localizes to the cell membrane and is also secreted; expressed primarily in natural killer cells and a subset of circulating monocytes. Related references: PMID:2526846 PMID:27670158
• Features immunoglobulin-like domains, disulfide bonds, glycosylation sites, and phosphorylation; identified as a reference proteome component.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide spanning amino acids 150–250 of human FCGR3A; consider performing peptide blocking experiments to confirm specificity in your assay system.
• For Western blot, a band near 29 kDa is expected. Validate detection using relevant cell lines known to express FCGR3A, such as NK cells, and optimize loading controls.
• This antibody cross-reacts with mouse and rat FCGR3A, which may enable interspecies studies. However, validation in the specific sample matrix is recommended.
• In ELISA applications, design coating antigens or capture antibodies that present the immunogenic region effectively, and include appropriate positive and negative controls.
• Given FCGR3A’s role in ADCC, this antibody may serve as a tool in Fc receptor engagement assays, but functional validation in your biological context is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials, covering every stage from concept to clinic. For FCGR3A-targeted studies in immunology and therapeutic antibody research, we offer a comprehensive portfolio including validated polyclonal and monoclonal antibodies, bulk ancillary reagents, and specialized support for rare target sourcing. Partner with CamelBio to secure reliable, performance-optimized raw materials and accelerate your assay development.