Anti-FCGR2B Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P31994

Core Product Specifications and Parameters

Biological Background: FCGR2B Function and Localization

• FCGR2B, also known as CD32, FcγRIIB, or CD32B, is a low-affinity receptor for the Fc region of complexed or aggregated immunoglobulin gamma (IgG). It is a member of the immunoglobulin superfamily.
• The receptor exists as multiple isoforms (e.g., IIB1 and IIB2) produced by alternative splicing. Isoform IIB1 lacks motifs for endocytosis and phagocytosis, while isoform IIB2 does not trigger phagocytosis upon ligand binding.
• FCGR2B plays a key immunoregulatory role by down-modulating cellular activation triggered through B-cell receptors (BCR), T-cell receptors (TCR), or other Fc receptors, thereby shaping antibody production and immune complex handling.
• It is primarily localized to the cell membrane as a single-pass type I membrane protein, with an extracellular region containing two immunoglobulin-like domains, a transmembrane helix, and a cytoplasmic tail.
• The protein is broadly distributed across myeloid and lymphoid lineages, including monocytes, neutrophils, macrophages, basophils, eosinophils, Langerhans cells, B-cells, platelets, and placental endothelial cells. It is not detected on natural killer cells.
• Post-translational modifications include N-linked glycosylation, disulfide bond formation, and phosphorylation, which regulate its activity and interactions.
• Genetic variations and chromosomal rearrangements involving FCGR2B have been linked to autoimmune conditions such as systemic lupus erythematosus (SLE).
• Additionally, FCGR2B acts as a proto-oncogene and has been implicated in host-virus interactions, highlighting its multifaceted role in immunity and disease.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 43–217 within the extracellular domain, targeting a region critical for ligand binding; consider verifying epitope accessibility in native versus denatured conditions for applications like IF-P versus WB.
• For western blotting, the predicted molecular weight is ~34 kDa, but actual bands may shift due to glycosylation; include proper positive and negative controls in your experimental design.
• In immunofluorescence on paraffin-embedded sections (IF-P), optimize antigen retrieval and permeabilization steps to ensure detection of the membrane-bound protein.
• When performing ELISA, evaluate cross-reactivity with other FcγR family members and validate specificity in the relevant sample matrix (e.g., serum, cell lysates).
• Given the broad cross-reactivity with human, mouse, and rat, confirm species-specific reactivity profiles in your assay system, especially when probing for low-abundance targets.
• As a polyclonal antibody, lot-to-lot consistency should be monitored; we recommend testing each new lot in a pilot study before large-scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consultancy—covering every stage from concept to clinic. This anti-FCGR2B antibody serves as a key reagent for investigations into immune regulation, autoimmune diseases, and Fc receptor biology. Our supply capabilities include validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your assay development and biomarker research.