Anti-FCGR2A Polyclonal Antibody for WB, IF-P, ELISA - P12318

Core Product Specifications and Parameters

Biological Background: FCGR2A Function and Localization

• FCGR2A encodes the low-affinity immunoglobulin gamma Fc region receptor II-a (FcγRIIa), also known as CD32 or CDw32, which belongs to the immunoglobulin superfamily of receptors.
• The receptor binds with low affinity to the Fc region of IgG antibodies, initiating cellular immune responses such as phagocytosis of opsonized antigens and clearance of immune complexes.
• FcγRIIa is expressed on the surface of immune cells, including monocytes, neutrophils, and eosinophil platelets, where it mediates antibody-dependent cellular cytotoxicity (ADCC) and cytokine release.
• Subcellularly, it localizes to the cell membrane as a single-pass type I transmembrane protein, with its extracellular immunoglobulin-like domains responsible for ligand binding.
• The protein undergoes post-translational modifications, including glycosylation and phosphorylation, which regulate its signaling through immunoreceptor tyrosine-based activation motifs (ITAMs) in its cytoplasmic domain.
• Alternative splicing of the FCGR2A gene generates multiple isoforms, and genetic polymorphisms (e.g., H131R) influence affinity for IgG subclasses and disease susceptibility.
• Keywords associated with the protein include disulfide bonds, signal peptide, and transmembrane helix, reflecting its structural features as a membrane-bound signaling receptor.

Experimental Guidance and Technical Tips

• The immunogen covers a large portion of the extracellular domain (aa 34-316), suggesting the antibody may recognize extracellular epitopes, making it suitable for live-cell IF or flow cytometry under native conditions.
• For Western blot, denaturing and reducing conditions may affect detection; a band around 35 kDa (predicted) is expected, but glycosylation may cause higher apparent molecular weight.
• In immunofluorescence, permeabilization may be required for intracellular staining, though the membrane localization should be considered; optimize fixation/permeabilization protocols.
• For ELISA, coating with recombinant FcγRIIa or cell lysates can be used; the antibody's polyclonal nature may provide high sensitivity.
• Cross-reactivity with mouse and rat is reported; validate in the relevant tissue/cell types, as sequence homology varies.

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CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For Fc receptor research or diagnostic applications, our portfolio includes validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. We also support rare target sourcing, ensuring seamless integration into your assay development pipeline.