Anti-FCGR1B Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q92637

Core Product Specifications and Parameters

Biological Background: FCGR1B Function and Localization

• FCGR1B, also known as CD64b or Fc gamma receptor IB, is a putative high affinity IgG Fc receptor. It is encoded by the FCGR1BP gene (FCGR1B/IGFRB) and shares structural features with other Fc gamma receptors.
• The protein contains immunoglobulin-like domains and belongs to the Fc receptor family, displaying sequence similarity to FCGR1A but with likely lower IgG binding affinity.
• FCGR1B is predicted to participate in the adaptive humoral immune response by binding the Fc region of immunoglobulins gamma.
• Alternative splicing has been reported, leading to multiple transcript variants that may produce distinct protein isoforms.
• Subcellular localization is at the cell membrane, anchored via a single-pass transmembrane helix.
• Post-translational modifications include N-linked glycosylation and disulfide bond formation, typical of Fc receptors.
• Key functional keywords include Adaptive immunity, IgG-binding protein, Immunoglobulin domain, Receptor, and Signal peptide.
• The calculated molecular weight is approximately 32 kDa (32,232 Da), consistent with the predicted mature protein.

Experimental Guidance and Technical Tips

• Immunogen design: A synthetic peptide spanning amino acids 100–200 of human FCGR1B (NP_001017986.1) was used; this region lies within the extracellular domain and may target the IgG-binding site.
• For Western blot (WB): Expect a primary band near 32 kDa. Due to alternative splicing, additional bands may be observed; validate in the relevant tissue or cell line.
• For immunohistochemistry on paraffin-embedded sections (IHC-P): Antigen retrieval and primary antibody dilution may require optimization for each sample type.
• For ELISA: Standard direct or indirect ELISA protocols can be employed; consider pairing with a matched secondary antibody for detection.
• As a rabbit polyclonal antibody, it recognizes multiple epitopes, potentially offering higher sensitivity but requiring careful blocking to minimize non-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers by providing validated antibody pairs and optimized polyclonal antibodies like anti-FCGR1B for immune-related assay development. Beyond antibodies, we offer bulk ancillary reagents and specialized sourcing for rare targets, ensuring your project moves from concept to clinic efficiently.