Anti-FCF1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9Y324

Core Product Specifications and Parameters

Biological Background: FCF1 Function and Localization

• FCF1 (rRNA-processing protein FCF1 homolog) is a component of the small subunit (SSU) processome, the earliest precursor of the small eukaryotic ribosomal subunit.
• It participates in ribosome biogenesis by facilitating pre-rRNA folding, modifications, rearrangements, and cleavage, as well as targeted degradation by the RNA exosome.
• The protein is localized to the nucleus, specifically the nucleolus, where ribosome assembly occurs.
• FCF1 is associated with rRNA processing and has been identified through proteomics studies.
• The gene is also known by aliases Bka, UTP24, CGI-35, and C14orf111.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 100-198 of human FCF1; this region may be conserved across species, allowing cross-reactivity with rat.
• For Western blot (WB), the predicted molecular weight is ~23 kDa; consider using appropriate controls to confirm specificity.
• In IHC-P and IF/ICC, nuclear/nucleolar staining is expected; validate in appropriate tissue or cell lines expressing FCF1.
• ELISA can be used for quantitative detection; optimize coating conditions and antibody dilution for best signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to validated IVD raw materials including antibodies, antigens, and ancillary reagents. This anti-FCF1 antibody serves ribosome biogenesis research and assay development. Our portfolio covers optimized monoclonal and polyclonal antibodies, bulk supply, and rare target sourcing to streamline your project from concept to clinic.