Anti-FCER1G Polyclonal Antibody for WB, ELISA - P30273

Core Product Specifications and Parameters

Biological Background: FCER1G Function and Localization

• FCER1G encodes the high affinity immunoglobulin epsilon receptor subunit gamma, also known as FcRgamma.
• It is an adapter protein containing an immunoreceptor tyrosine-based activation motif (ITAM) that transduces activation signals from various immunoreceptors.
• As a component of the high-affinity IgE receptor, it mediates allergic inflammatory signaling in mast cells.
• It associates with pattern recognition receptors CLEC4D and CLEC4E to form signaling complexes in myeloid cells.
• Binding of mycobacterial trehalose 6,6'-dimycolate (TDM) to the receptor complex triggers ITAM phosphorylation, activating SYK, CARD9 and NF-kappa-B.
• It is involved in integrin-mediated neutrophil and platelet activation.
• Subcellularly located at the cell membrane.
• Post-translational modifications include signal peptide cleavage, disulfide bond formation, and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 19-86 of human FCER1G, covering the predicted extracellular domain. This design may favor detection of the mature, glycosylated form.
• For Western blotting, consider using a 12-15% gel to resolve the ~10 kDa protein band, and include reducing agents to break disulfide-linked dimers.
• For ELISA, ensure coating buffer and detection conditions are optimized for recombinant protein or peptide antigens.
• Validate the antibody in the relevant sample system, including positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research teams with reliable access to IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies like this FCER1G rabbit pAb. Our one-stop supply covers bulk ancillary reagents and rare-target sourcing, helping you accelerate allergy and immunology assay development from concept to clinic.