Anti-FcεR1α Rabbit Monoclonal Antibody for WB, FC, ELISA - P12319

Core Product Specifications and Parameters

Biological Background: FcεR1α Function and Localization

• FcεR1α is the high-affinity receptor for IgE, also designated Fc-epsilon RI-alpha or FcERI, encoded by the FCER1A gene.
• It mediates IgE effector functions in myeloid cells; upon IgE binding and cross-linking by allergen, it initiates signaling cascades that lead to myeloid cell activation and degranulation.
• On mast cells, basophils, and eosinophils, it stimulates the secretion of vasoactive amines, lipid mediators, and cytokines, contributing to allergic inflammation and host defense against helminths, bacteria, and venom.
• Subcellularly, FcεR1α is a single-pass type I transmembrane protein localized to the cell membrane.
• It is a glycoprotein with immunoglobulin-like domains and disulfide bonds, and its dysregulation can trigger life-threatening anaphylactic reactions.
• Expressed in eosinophils, it plays a pivotal role in immediate hypersensitivity and anti-microbial immunity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 26–205), which may facilitate recognition of the native surface receptor in applications such as flow cytometry.
• For Western blot, a band near 30 kDa is expected based on the predicted molecular weight; validate the observed size in your sample system.
• For flow cytometry, consider using a relevant cell line or primary cells known to express FcεR1α, such as eosinophils, with appropriate isotype controls.
• ELISA performance may be influenced by coating conditions and detection format; perform initial checkerboard titrations to optimize signal-to-noise ratios.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to a comprehensive portfolio of IVD raw materials. For FcεR1α-related projects, we supply validated monoclonal antibodies, recombinant proteins, and ancillary reagents in bulk, alongside specialized services for rare target sourcing. Our integrated approach supports the development of robust allergy and inflammation diagnostics, from early feasibility to clinical application.