Anti-FceR1 alpha Monoclonal Antibody for WB, ELISA - P12319

Core Product Specifications and Parameters

Biological Background: FceR1 alpha Function and Localization

• Identifies as the alpha subunit of the high-affinity IgE receptor (FcεRI), also known as Fc-epsilon RI-alpha, FCE1A, FcERI, or FCERIA.
• Binds immunoglobulin E (IgE) with high affinity, initiating signaling cascades on mast cells, basophils, and eosinophils upon allergen cross-linking.
• Triggers degranulation and release of vasoactive amines, lipid mediators, and cytokines, driving immediate hypersensitivity and allergic inflammation.
• Functions in host defense against helminth parasites, pathogenic bacteria, and venom toxicity.
• Localizes to the cell membrane as a single-pass transmembrane protein.
• Expressed in eosinophils, with relevance in allergic responses and chronic inflammation.
• Undergoes post-translational modifications including disulfide bond formation and glycosylation, contributing to receptor stability and ligand binding.
• Dysregulated signaling can lead to life-threatening anaphylactic reactions, underscoring its role as a therapeutic target.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (amino acids 26–205) of human FceR1 alpha, suggesting the antibody may recognize both native and denatured forms. For WB, consider reducing SDS-PAGE and transfer conditions, validating with appropriate controls.
• For ELISA, ensure the coating antigen presents the relevant epitope; inclusion of a blocking step with non-fat milk or BSA may reduce background.
• As reactivity is reported against human, mouse, and rat, validate species-specific detection in relevant sample systems such as eosinophil lysates or mast cell lines.
• The reported protein weight is 30 kDa (UniProt predicts ~29.6 kDa, possibly reflecting glycosylation); a band shift may be observed depending on sample preparation.

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CamelBio provides streamlined access to high-quality IVD raw materials for immunology and allergy research, including validated monoclonal antibodies like this anti-FceR1 alpha clone. Our portfolio extends to optimized antibody pairs, bulk reagents, and rare-target sourcing to support your diagnostic development from concept to clinical application.