Anti-FCAR Polyclonal Antibody for WB, IF/ICC, ELISA - P24071

Core Product Specifications and Parameters

Biological Background: FCAR (CD89) Function and Localization

• FCAR, also known as CD89, is a type I transmembrane glycoprotein and the receptor for immunoglobulin alpha (IgA), belonging to the immunoglobulin superfamily.
• The protein mediates immune effector functions such as cytokine production, phagocytosis, and degranulation upon binding to IgA immune complexes.
• FCAR is expressed differentially in myeloid cells: monocytes express all three major isoforms (A.1, A.2, A.3), while alveolar macrophages typically express only one surface isoform.
• Subcellular localization includes the cell membrane; additionally, secreted forms can be generated by alternative splicing or proteolytic shedding.
• Key structural features include two extracellular Ig-like domains, a single transmembrane helix, and a short cytoplasmic tail lacking classical signaling motifs; signaling occurs through association with the common FcR γ-chain.
• Post-translational modifications include N-linked glycosylation at multiple sites and disulfide bond formation, contributing to stability and ligand binding.
• Functional studies implicate FCAR in both systemic and mucosal immunity, with roles in inflammatory responses and regulation of immune homeostasis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 60-287), which is predicted to be well-exposed for detection. For Western blot, consider that the observed molecular weight may be higher (~45-65 kDa) due to glycosylation.
• For IF/ICC, use permeabilization if targeting the cytoplasmic tail or if the isoform is predominantly intracellular; for surface staining, live-cell labeling with the antibody may be feasible.
• Since FCAR is expressed in primary myeloid cells, relevant positive control samples include human peripheral blood mononuclear cells (PBMCs) or mouse spleen lysates.
• ELISA applications may be optimized using recombinant FCAR-Fc fusion proteins as capture/standard; note that the antibody detects both membrane-bound and soluble forms.
• Cross-reactivity with mouse is reported; however, sequence homology in the extracellular domain is only about 50%, so validate in your specific mouse strain and tissue.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw-material procurement, offering validated antibody pairs, bulk reagents, and custom polyclonal antibody development. For immunology and immune-receptor research, we supply a wide range of antibodies against Fc receptors and immune checkpoint molecules. Count on CamelBio to streamline your sourcing process from discovery to diagnostic implementation.