Anti-FBXW8 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q8N3Y1

Core Product Specifications and Parameters

Biological Background: FBXW8 Function and Localization

• FBXW8 functions as a substrate-recognition component of the Cul7-RING(FBXW8) ubiquitin ligase complex, mediating ubiquitination and subsequent proteasomal degradation of target proteins.
  Related references: PMID:17205132 PMID:18498745 PMID:21572988
• It catalyzes ubiquitination of GORASP1, thereby regulating Golgi morphogenesis and dendrite patterning in the brain.
  Related references: PMID:21572988
• In an mTOR-dependent manner, it recognizes phosphorylated IRS1 and targets it for degradation.
  Related references: PMID:18498745
• It ubiquitinates autophosphorylated MAP4K1/HPK1, influencing cell proliferation and differentiation.
  Related references: PMID:24362026
• FBXW8 also ubiquitinates phosphorylated cyclin-D1 (CCND1), though it is not a major regulator of CCND1 stability during the G1/S transition.
  Related references: PMID:17205132
• As an associated component of the 3M complex, FBXW8 contributes to the complex's functions.
  Related references: PMID:24793695
• The protein localizes primarily to the cytoplasm, perinuclear region, and Golgi apparatus, and features multiple WD40 repeat domains.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 500–598 of human FBXW8; for immunodetection, consider optimizing antibody dilution based on sample type and detection system.
• For Western blotting, a band at ~67 kDa is expected; include appropriate positive and negative controls, and validate with known expressing cell lines.
• In immunofluorescence/ICC, subcellular localization to the perinuclear/Golgi region can be assessed; fixation and permeabilization methods may require optimization.
• For ELISA, use the immunogen peptide or recombinant protein as a standard, and titrate the antibody to achieve optimal sensitivity.
• Cross-reactivity with mouse and rat allows for use in rodent models; however, confirm specificity in the relevant experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing platform for IVD raw materials, supporting diagnostic developers and research institutes with validated antibodies, bulk reagents, and technical consultation. For ubiquitin-proteasome pathway investigations, including F-box protein research, explore our catalog of polyclonal and monoclonal antibodies as well as matched antigen standards. Whether you need rare-target raw materials or ancillary reagents for assay development, CamelBio aims to streamline your procurement from concept to clinic.