Anti-FBXW7 Polyclonal Antibody for WB, IHC-P, ELISA - Q969H0

Core Product Specifications and Parameters

Biological Background: FBXW7 Function and Localization

• FBXW7 (F-box/WD repeat-containing protein 7) is the substrate recognition component of the SCF (SKP1-CUL1-F-box) E3 ubiquitin-protein ligase complex, which targets phosphorylated proteins for ubiquitination and proteasomal degradation. Related references: PMID:17434132, PMID:22748924, PMID:26976582
• It recognizes specific phosphodegrons on substrates such as cyclin E, MYC, NOTCH1 intracellular domain, JUN, and MCL1, thereby regulating cell cycle progression, apoptosis, and differentiation. Related references: PMID:11565034, PMID:11585921, PMID:12354302
• FBXW7 acts as a negative regulator of JNK signaling by mediating ubiquitin-dependent degradation of phosphorylated JUN. Related references: PMID:14739463
• It participates in circadian clock control by promoting ubiquitination and proteasomal degradation of the transcriptional repressor NR1D1, thus influencing hepatic lipid and glucose metabolism. Related references: PMID:27238018
• In response to DNA double-strand breaks, ATM phosphorylation drives FBXW7 localization to damage sites and mediates 'Lys-63'-linked ubiquitination of XRCC4, enhancing non-homologous end joining repair. Related references: PMID:26774286
• FBXW7 is involved in bone homeostasis and negatively regulates osteoclast differentiation. Related references: PMID:29149593
• It localizes predominantly to the nucleus (nucleoplasm, nucleolus) and chromosomes, with some cytoplasmic presence.
• Widely expressed, including in brain, and associated with disease variants and intellectual disability; multiple isoforms arise from alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 231–371 of human FBXW7 (NP_361014.1). This N‑terminal region may be conserved across isoforms; check sequence homology for your target variant.
• In Western blot, expect a band near 80 kDa. Use appropriate protein ladders and validate with positive controls (e.g., human, mouse, or rat cell lysates).
• For IHC‑P, antigen retrieval and antibody dilution should be optimized empirically. Confirm staining specificity with known positive and negative tissues or siRNA‑treated samples.
• ELISA performance depends on plate coating and detection format. Consider checkerboard titrations for optimal signal‑to‑noise ratio.
• Cross‑reactivity is reported for human, mouse, and rat. When working with other species, align the immunogen sequence to verify potential recognition.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research labs with a broad portfolio of validated IVD raw materials, including polyclonal and monoclonal antibodies, antigen pairs, and ancillary reagents. This FBXW7 antibody supports ubiquitin‑proteasome pathway studies and cancer research, reflecting our commitment to supplying high‑quality immunological tools. Whether you need bulk quantities, rare‑target sourcing, or technical consultancy from concept to clinical application, CamelBio offers reliable, end‑to‑end support.