Anti-FBXW5 Polyclonal Antibody for WB, ELISA - Q969U6

Core Product Specifications and Parameters

Biological Background: FBXW5 Function and Localization

• FBXW5 (F-box/WD repeat-containing protein 5) is also known as F-box and WD-40 domain-containing protein 5, encoded by the FBXW5 (FBW5) gene.
• Serves as the substrate recognition component of both SCF (SKP1-CUL1-F-box protein) and DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes, directing specific proteins for ubiquitination and proteasomal degradation.
• In the SCF(FBXW5) complex, mediates ubiquitination and degradation of SASS6 during S phase, which prevents centriole reduplication, and also targets EPS8 for degradation during G2 phase to enable mitotic cell shape changes.
• As part of the DCX(FBXW5) complex, mediates polyubiquitination and subsequent degradation of TSC2, suggesting a role in mTOR pathway regulation.
• May function as a negative regulator of MAP3K7/TAK1 signaling in the interleukin-1B (IL1B) pathway, linking ubiquitin-mediated proteolysis to inflammatory responses.
• Localizes to the cytoplasm according to experimental evidence.
• Annotated with keywords including Alternative splicing, Phosphoprotein, and WD repeat, indicating structural motifs and potential regulatory modifications.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 184-566 of human FBXW5, covering the C-terminal WD repeat domain; consider this when assessing isoform reactivity or domain-specific binding.
• For Western blot, validate the antibody with a positive control lysate (e.g., human cell lines) and use appropriate molecular weight markers around 64 kDa.
• ELISA applications may require optimizing coating conditions and detection reagents; consider recombinant FBXW5 protein as a standard.
• Since cross-reactivity is listed for human only, verify performance in other species if needed, as sequence homology outside the immunogen region may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies IVD raw material procurement for diagnostic manufacturers, labs, and research institutes by offering validated antibodies like this FBXW5 polyclonal antibody, which supports ubiquitin ligase and centriole biology studies. As a comprehensive sourcing partner, we supply optimized antibody pairs, bulk ancillary reagents, and can assist in identifying rare targets—helping you move from concept to clinic with reliable, research-grade materials.