Anti-FBXW2 Rabbit Polyclonal Antibody for WB, ELISA - Q9UKT8

Core Product Specifications and Parameters

Biological Background: FBXW2 Function and Localization

• FBXW2, also known as protein MD6, is an F-box/WD repeat-containing protein 2 that belongs to the F-box protein family.
• It functions as the substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex, directing target proteins for ubiquitination and proteasomal degradation.
• The protein features WD repeats and an F-box domain, which mediate protein-protein interactions within the ubiquitin ligase complex.
• Alternative splicing of the FBXW2 gene produces multiple isoforms, potentially expanding its regulatory roles.
• Acetylation has been identified as a post-translational modification by proteomics, suggesting additional regulatory layers.
• FBXW2 is involved in the ubiquitin-proteasome system, a critical pathway for maintaining cellular protein homeostasis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 102–454 of human FBXW2, which may allow detection of multiple isoforms; confirm specificity in your target system.
• For Western blotting, consider using standard protocols with a starting dilution series to optimize signal-to-noise ratio for your specific samples.
• In ELISA, test both direct and capture formats to determine optimal antibody performance with your antigen.
• Validate cross-reactivity in mouse and rat samples using appropriate tissue or cell lysates, as reactivity may vary across species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic and research laboratories with integrated supply and technical consultation for IVD raw materials. For studies on the ubiquitin-proteasome system, we offer validated antibody pairs, optimized polyclonal antibodies like this anti-FBXW2 reagent, bulk ancillary reagents, and rare target raw-material sourcing. Our one-stop model helps streamline procurement and accelerate assay development from concept to clinic.