Anti-FBXW11 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9UKB1

Core Product Specifications and Parameters

Biological Background: FBXW11 Function and Localization

• FBXW11 (also known as BTRCP2, FBW1B, HOS) is a member of the F-box/WD repeat protein family and serves as the substrate recognition component of the SCF (SKP1-CUL1-F-box) E3 ubiquitin ligase complex. Related references: PMID:10437795, PMID:10648623, PMID:11158290
• The SCF(FBXW11) complex mediates ubiquitination and proteasomal degradation of key signaling molecules, including β-catenin (CTNNB1), IκBα (NFKBIA), and NFKB1 p105, thereby regulating Wnt signaling and NF-κB activation. Related references: PMID:10321728, PMID:10437795, PMID:10644755, PMID:11158290
• It controls cell cycle progression by targeting CDC25A for degradation, contributing to the intra-S-phase DNA damage checkpoint response. Related references: PMID:14603323
• FBXW11 plays an essential role in circadian rhythm control by degrading phosphorylated PER1 and PER2, thus influencing clock-dependent transcription. Related references: PMID:15917222
• The protein localizes to both the cytoplasm and nucleus, consistent with its involvement in diverse signaling pathways.
• Additional functions include regulation of mTORC1 signaling via DEPTOR, TFE3, and MITF degradation, as well as a role in centriole separation during mitosis through CEP68 ubiquitination. Related references: PMID:22017875, PMID:22017876, PMID:36608670, PMID:25503564
• Keywords associated with FBXW11 include Ubl conjugation pathway, Wnt signaling pathway, host-virus interaction, and disease variant, reflecting its broad biological significance.
• During HIV-1 infection, the viral protein Vpu hijacks FBXW11 to ubiquitinate and deplete the antiviral restriction factor BST2, aiding viral escape.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide of human FBXW11; the resulting polyclonal antibody can recognize multiple epitopes, which may reduce the impact of minor sequence variations.
• For Western blot analysis, a band around 62 kDa is expected; include appropriate positive and negative controls, and validate the antibody in the relevant sample system (e.g., human, mouse, rat lysates).
• In immunofluorescence/immunocytochemistry, consider optimizing fixation and permeabilization protocols to detect FBXW11 in both cytoplasmic and nuclear compartments.
• For ELISA, perform standard optimization steps such as checkerboard titration of coating antigen and antibody concentrations to achieve desired sensitivity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials, fulfilling needs from validated antibody pairs to bulk ancillary reagents. This FBXW11 antibody supports research in ubiquitin-proteasome pathways and signal transduction. Leverage CamelBio's expertise for rare target sourcing and one-stop procurement solutions.