Anti-FBXO7 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9Y3I1

Core Product Specifications and Parameters

Biological Background: FBXO7 Function and Localization

• FBXO7 (F-box only protein 7), also known as FBX, FBX7, PKPS, PARK15, is a substrate recognition component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex, playing roles in cell cycle, proliferation, and chromosome stability. Related references: PMID:15145941 PMID:34791250
• It mediates ubiquitination and proteasomal degradation of target proteins including BIRC2 and DLGAP5. Related references: PMID:15145941 PMID:16510124 PMID:22212761
• FBXO7 functions in mitophagy downstream of PINK1, targeting PRKN to damaged mitochondria and promoting MFN1 ubiquitination.
• It inhibits NF-kappa-B signaling by promoting ubiquitination of UXT isoform 2 and TRAF2. Related references: PMID:33010352 PMID:22212761
• FBXO7 regulates proteasome assembly and activity by ubiquitinating the PSMA2 subunit with Lys-63-linked polyubiquitin chains.
• It promotes Lys-48-linked polyubiquitination of SIRT7, leading to hydrogen peroxide-induced cell death. Related references: PMID:36646384
• FBXO7 localizes to the cytoplasm, nucleus, mitochondrion, and cytosol.
• Mutations in FBXO7 are associated with Parkinson disease 15 (PARK15), and the protein is linked to neurodegeneration.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 353-522) of human FBXO7. This region may detect full-length protein and some isoforms; confirm specificity using appropriate controls.
• For Western blotting (WB), a band around 59kDa is expected; however, post-translational modifications may cause apparent molecular weight shifts.
• Polyclonal antibodies may exhibit lot-to-lot variability; consider titrating the antibody for each new lot and application.
• The antibody is reported to detect human, mouse, and rat samples; for other species, in-silico sequence alignment can guide cross-reactivity assessment.
• For immunofluorescence (IF/ICC), optimize fixation and permeabilization conditions.
• For ELISA, pair with a suitable secondary antibody and validate using recombinant protein or cell lysates.
• Due to the role of FBXO7 in mitophagy, consider stimulation protocols to induce mitochondrial damage for functional studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with comprehensive access to IVD raw materials. This anti-FBXO7 antibody supports studies in ubiquitin pathways, neurodegeneration, and cancer biology. From validated antibody pairs to bulk reagents and rare target sourcing, we streamline your assay development journey from concept to clinic.