Anti-FBXO42 Polyclonal Antibody for WB - Q6P3S6

Core Product Specifications and Parameters

Biological Background: FBXO42 Function and Localization

• FBXO42, also known as F-box only protein 42 or Just one F-box and Kelch domain-containing protein (JFK), is a member of the F-box protein family.
• It functions as the substrate-recognition component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex.
• FBXO42 specifically recognizes and targets the tumor suppressor p53/TP53 for ubiquitination and subsequent proteasomal degradation.
• The protein contains characteristic Kelch repeat domains, which are likely involved in substrate binding.
• FBXO42 is a phosphoprotein, suggesting that its activity or stability may be regulated by phosphorylation.
• The gene encoding FBXO42, designated FBXO42 (also known as FBX42, JFK, KIAA1332), is the sole family member described in the current record.
• The predicted molecular weight of FBXO42 is approximately 78 kDa (UniProt: 77839 Da).
• Information on subcellular localization and tissue specificity is not available from the current UniProt entry.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 468-717 of human FBXO42, covering a C-terminal region. Consider the possibility that the antibody may not recognize N-terminal isoforms or fragments.
• For Western blot (WB), validate detection of full-length FBXO42 at ~78 kDa in lysates from human, mouse, or rat cells. Include appropriate controls to confirm specificity.
• For immunohistochemistry (IHC-P), antigen retrieval conditions should be optimized for the specific tissue and fixation method.
• For ELISA, use recombinant FBXO42 protein or peptide as a standard to establish assay sensitivity and linearity.
• Pre-adsorption with the immunogen may be used to confirm signal specificity in all applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive supplier of IVD raw materials, offering a one-stop bridge from concept to clinic for diagnostic manufacturers and research laboratories. This anti-FBXO42 polyclonal antibody supports investigations into ubiquitin-proteasome pathways and p53 regulation. Beyond this product, CamelBio provides validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing of rare targets to streamline your assay development and production needs.