Anti-FBXO42 Polyclonal Antibody for WB, ELISA - Q6P3S6

Core Product Specifications and Parameters

Biological Background: FBXO42 Function and Localization

• FBXO42, also known as JFK, Fbx42, or Just one F-box and Kelch domain-containing protein, is a member of the F-box protein family.
• It functions as the substrate-recognition component of SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complexes.
• Specifically recognizes the tumor suppressor p53/TP53, promoting its ubiquitination and subsequent proteasomal degradation.
• Contains characteristic Kelch repeat domains, which are likely involved in substrate binding or protein-protein interactions.
• Classified under keywords: Kelch repeat, Phosphoprotein, Ubiquitin conjugation pathway, indicating its role in post-translational modification and protein turnover.
• The protein is a reference proteome component and has been identified in proteomics studies.
• Molecular weight is approximately 78 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 468-717, covering the C-terminal region that includes Kelch repeats; this design may target specific isoforms or functional domains. Consider validating in the relevant sample system.
• For Western blot (WB), a band around 78 kDa is expected; validate under denaturing and reducing conditions, and include appropriate controls.
• The antibody is also suitable for ELISA; optimal dilutions should be determined experimentally.
• Cross-reactivity with mouse is reported; confirm with mouse lysates if working with murine models.
• When studying FBXO42-mediated p53 degradation, include proteasome inhibitor controls to assess ubiquitination effects.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies like this anti-FBXO42 antibody, and ancillary reagents for ubiquitin-proteasome pathway research. Our supply-chain expertise spans bulk custom antibodies, rare target sourcing, and technical consulting to accelerate your assay development from concept to clinic.