Anti-FBXO4 Rabbit Polyclonal Antibody for WB, ELISA - Q9UKT5

Core Product Specifications and Parameters

Biological Background: FBXO4 Function and Localization

• FBXO4, also known as FBX4, is an F-box only protein encoded by the FBXO4 gene.
• It serves as the substrate recognition subunit of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Related references: PMID:10531035, PMID:18598945, PMID:20181953
• FBXO4 promotes the ubiquitination and proteasomal degradation of cyclin D1 (CCND1). Related references: PMID:18598945
• It also targets telomeric repeat-binding factor 1 (TERF1) for ubiquitination. Related references: PMID:16275645, PMID:20159592
• Upon phosphorylation by GSK3B, FBXO4 ubiquitinates FXR1, leading to its degradation by the proteasome. Related references: PMID:29142209
• FBXO4 localizes to the cytoplasm.
• The protein is subject to phosphorylation and has multiple isoforms due to alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 138–387 of human FBXO4, covering a region that includes part of the F-box domain and C-terminal sequences. This design is likely to recognize both full-length and potential splice variants.
• For WB, the predicted molecular weight is approximately 44 kDa. Use appropriate controls and optimize antibody dilution for your specific experimental conditions.
• Cross-reactivity with mouse has been reported, making this antibody suitable for mouse model studies. Validate in your mouse tissue or cell line of interest.
• Recommended applications are WB and ELISA. For other applications, perform pilot experiments to determine feasibility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. We support researchers and diagnostic manufacturers in the ubiquitin-proteasome system field, providing one-stop sourcing from concept to clinic. For FBXO4-related research, we can assist with custom antibody development and rare target raw-material procurement to accelerate your assay development.