Anti-FBXO32 Rabbit Polyclonal Antibody for IF/ICC, IF-P, ELISA - Q969P5

Core Product Specifications and Parameters

Biological Background: FBXO32 Function and Localization

• Encoded by the FBXO32 gene, F-box only protein 32 (also known as Atrogin-1 and muscle atrophy F-box protein, MAFbx) is a 42 kDa member of the F-box protein family. Multiple isoforms are produced by alternative splicing.
• FBXO32 functions as the substrate recognition component of an SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex, mediating the ubiquitination and subsequent proteasomal degradation of target proteins during skeletal muscle atrophy.
• It specifically recognizes and binds to phosphorylated target proteins, playing a central role in muscle protein degradation pathways associated with atrophy.
• The protein negatively regulates macrophage efferocytosis by promoting ubiquitination of the transcription factor KLF4, thereby suppressing transcription of the receptor tyrosine kinase MERTK (By similarity).
• FBXO32 regulates LPS-induced apoptosis and mitochondrial dysfunction through ubiquitination and degradation of ANXA1 (By similarity).
• It also mediates cyclin D1 protein stabilization via 'Lys-27'-linked ubiquitination. Related references: PMID:40307251
• Subcellularly, FBXO32 is found in both the cytoplasm and nucleus, consistent with its roles in protein turnover and gene regulation.
• Expression is highly specific to cardiac and skeletal muscle, underscoring its specialized function in muscle tissue dynamics.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 201-300 of human FBXO32; epitope recognition may be influenced by protein conformation or post-translational modifications in different sample preparations.
• For immunofluorescence (IF/ICC and IF-P) applications, consider appropriate fixation and permeabilization conditions to preserve endogenous FBXO32 localization in muscle cell lines or tissue sections.
• ELISA assays can be designed with appropriate blocking and detection reagents, and it is advisable to validate the antibody's performance in the relevant sample system using a control antigen.
• When working with mouse or rat samples, confirm cross-reactivity and signal specificity under your experimental conditions due to possible species differences in the target sequence.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. This FBXO32 polyclonal antibody supports research into muscle atrophy, ubiquitination pathways, and related disease mechanisms—areas where reliable raw materials are critical. Beyond this product, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your assay development from concept to clinic.