Anti-FBXO32 (Fbx32, MAFbx) Rabbit Polyclonal Antibody - Q969P5

Core Product Specifications and Parameters

Biological Background: FBXO32 Function and Localization

• FBXO32 (F-box only protein 32), also known as Atrogin-1 or muscle atrophy F-box protein (MAFbx), is the substrate recognition component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex.
• It specifically marks phosphorylated targets, such as TERF1, for ubiquitination and proteasomal degradation, playing a key role in skeletal muscle atrophy.
• FBXO32 negatively regulates macrophage efferocytosis by ubiquitinating the transcription factor KLF4, thereby suppressing MERTK receptor tyrosine kinase transcription (By similarity).
• It regulates lipopolysaccharide (LPS)-induced apoptosis and mitochondrial dysfunction through the ubiquitination and degradation of annexin A1 (ANXA1) (By similarity).
• FBXO32 mediates cyclin D1 protein stabilization via 'Lys-27'-linked ubiquitination. Related references: PMID:40307251
• Primarily localized in the cytoplasm and nucleus, with specific expression in cardiac and skeletal muscle.
• The gene undergoes alternative splicing and is linked to disease variants, highlighting its importance in ubiquitin conjugation pathways.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 206–355 in the C-terminal region, potentially detecting full-length FBXO32 and relevant isoforms.
• For IF/ICC, consider optimising fixation and permeabilisation to preserve the antigen, especially in muscle tissue sections.
• Validate antibody specificity in relevant positive controls such as skeletal or cardiac muscle lysates or cell lines expressing FBXO32.
• ELISA sensitivity may require titration of both the capture antibody and antigen; pre-coating conditions should be optimised.
• Cross-reactivity with mouse and rat suggests suitability for translational studies, but confirm sequence homology in the target region for your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to IVD raw materials and technical services—from early R&D through clinical application. This anti-FBXO32 polyclonal antibody supports muscle atrophy and ubiquitination research, and we can supply validated antibody pairs, bulk ancillary reagents, and rare-target sourcing to accelerate your assay development.