Anti-FBXO31 Polyclonal Antibody for WB - Q5XUX0

Core Product Specifications and Parameters

Biological Background: FBXO31 Function and Localization

• Functions as a substrate-recognition component of the SCF(FBXO31) E3 ubiquitin ligase complex, mediating ubiquitination of C-terminally amidated proteins in oxidative stress (PubMed:39880951). Related references: PMID:39880951
• Promotes G1 arrest upon DNA damage by targeting phosphorylated cyclin D1 (CCND1) for degradation (PubMed:19412162, PubMed:29279382). Related references: PMID:19412162 PMID:29279382
• Crucial for p53/TP53-mediated DNA damage response by directing ubiquitination and degradation of phosphorylated MDM2 (PubMed:26124108). Related references: PMID:26124108
• Maintains genomic integrity by degrading cyclin A (CCNA1/CCNA2) during G1 phase (PubMed:31413110). Related references: PMID:31413110
• Targets an array of substrates including FBXO46, MAP2K6, CDT1, DUSP6, OGT, and PARD6A, thereby modulating cell cycle progression, DNA replication, and stress responses (PubMed:24936062, PubMed:30171069, PubMed:24828503, PubMed:23469015, PubMed:34686346, PubMed:39894887). Related references: PMID:24936062 PMID:30171069 PMID:24828503 PMID:23469015 PMID:34686346 PMID:39894887
• Localizes to the cytoplasm and centrosome.
• Highly expressed in the brain; mutations are associated with intellectual disability, and the protein undergoes alternative splicing and phosphorylation.

Experimental Guidance and Technical Tips

• For Western blotting (WB), optimize antibody dilution using lysates from FBXO31-expressing cells. Validate specificity via siRNA knockdown or overexpression controls, given the polyclonal nature of the antibody.
• The antibody has shown cross-reactivity with mouse and rat; consider using these species for in vivo model validation.
• In ELISA, recombinant FBXO31 protein or the immunogen fragment (aa 240-539) can serve as a positive control.
• Since this is a polyclonal antibody, lot-to-lot consistency should be assessed by the end user for critical quantitative assays.
• For cell cycle or DNA damage studies, treat cells with genotoxic agents (e.g., etoposide or UV) to induce FBXO31 substrate degradation and confirm functional activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to IVD raw materials, technical services, and consulting—from concept to clinic. This FBXO31 polyclonal antibody supports research into DNA damage response, cell cycle regulation, and ubiquitin-proteasome pathways. Beyond this product, CamelBio can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and facilitate rare target raw-material sourcing to accelerate your IVD assay development.