Anti-FBXO28 Rabbit Polyclonal Antibody for WB, ELISA - Q9NVF7

Core Product Specifications and Parameters

Biological Background: FBXO28 Function and Localization

• Acts as a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex, targeting TOP2A, RAB27A, or itself for ubiquitination and proteasomal degradation. Related references: PMID:27754753 PMID:31678254
• Regulates topoisomerase IIalpha (TOP2A) decatenation activity and plays a critical role in maintaining genomic stability. Related references: PMID:27754753
• Participates in lipid metabolism and inflammation by mediating ubiquitinated degradation of RAB27A (By similarity).
• Strongly regulates beta-cell survival without significantly affecting insulin secretion. Related references: PMID:29587369
• Essential for spindle morphology and actin-based spindle migration, likely through the ARPC2/ARP3 signaling pathway (By similarity).
• Localizes to the centromere, kinetochore, and nucleus, consistent with its functions in chromosome dynamics and cell division.
• Contains a characteristic F-box domain that mediates interaction with SKP1, linking substrates to the SCF ubiquitin ligase complex.
• The gene undergoes alternative splicing, potentially producing protein isoforms with distinct regulatory properties.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 91–312 of human FBXO28 (NP_055991.1), a region likely conserved across isoforms; polyclonal antibodies may recognize multiple splice variants.
• For Western blotting, an expected band near 41 kDa (calculated molecular weight) is typical; however, verify band specificity in the relevant sample system as post-translational modifications or isoforms may shift mobility.
• In ELISA, consider using recombinant FBXO28 or cell lysates with appropriate negative controls, and optimize coating/detection conditions for your assay.
• Cross-reactivity with human and rat has been reported; for other species, validation is recommended due to potential sequence divergence.
• Because FBXO28 is involved in ubiquitin-proteasome pathways, treating cells with proteasome inhibitors (e.g., MG132) may stabilize target proteins and enhance detection of ubiquitination intermediates if studied.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic manufacturers and research institutes, offering one-stop access to IVD raw materials, technical services, and consulting from concept to clinic. For FBXO28-centered research, we supply validated antibody pairs, recombinant proteins, and bulk ancillary reagents to streamline your assay development. Explore our portfolio for rare-target antibodies and let our team assist with customized sourcing solutions to meet your unique project needs.