Anti-FBXO22 Rabbit Polyclonal Antibody for WB, ELISA - Q8NEZ5

Core Product Specifications and Parameters

Biological Background: FBXO22 Function and Localization

• FBXO22 (F-box only protein 22, also known as FBX22 or FISTC1) is a substrate-recognition component of the SCF E3 ubiquitin ligase complex involved in ubiquitin-mediated proteolysis.
• It promotes degradation of sarcomeric proteins alpha-actinin (ACTN2) and filamin-C (FLNC), which is critical for cardiac and skeletal muscle contractile function.
• FBXO22 controls histone H3K9 and H3K36 methylation by regulating the stability of the histone demethylase KDM4A. Related references: PMID:21768309
• Through KDM4A, it targets methylated TP53 for degradation, influencing cellular senescence. Related references: PMID:26868148
• Under oxidative stress, FBXO22 ubiquitinates and promotes degradation of BACH1, relieving transcriptional repression. Related references: PMID:39504958
• It mediates 'Lys-27'-linked ubiquitination of MTOR upon amino acid starvation, inhibiting mTORC1 signaling. Related references: PMID:37979583
• FBXO22 inhibits SARS-CoV-2 replication by targeting viral NSP5 for degradation. Related references: PMID:39223933
• Subcellularly, it localizes to the cytoplasm, nucleus, and sarcomere Z-line; predominantly expressed in liver and cardiac muscle.

Experimental Guidance and Technical Tips

• For Western blot, expect a band at approximately 45 kDa; validate using lysates from human cell lines or tissues known to express FBXO22 (e.g., liver, cardiac muscle).
• The immunogen spans amino acids 104–403, covering a region that may detect multiple isoforms or splice variants; consider this when interpreting band patterns.
• In ELISA, optimize antigen coating (recombinant protein or peptide) and antibody dilution using a standard curve for quantitation.
• Due to the polyclonal nature, lot-to-lot variability can occur; always validate in your specific assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, connecting diagnostic manufacturers, laboratories, and research institutes with high-quality reagents and technical services from concept to clinic. This anti-FBXO22 rabbit polyclonal antibody is ideally positioned for research into ubiquitin-mediated degradation, muscle biology, and viral host interactions. We facilitate supply of validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare‑target raw‑material sourcing to accelerate your project’s development.