Anti-FBXO21 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O94952

Core Product Specifications and Parameters

Biological Background: FBXO21 Function and Localization

• FBXO21 (F-box only protein 21) is a member of the F-box protein family, defined by the presence of an F-box domain that facilitates interaction with SKP1 within SCF (SKP1-CUL1-F-box) E3 ubiquitin ligase complexes.
• It functions as a substrate-recognition component of SCF-type E3 ubiquitin ligases, directing specific proteins for ubiquitination and subsequent proteasomal degradation.
• The FBXO21 gene undergoes alternative splicing, producing multiple transcript variants that encode distinct protein isoforms, though their individual roles remain poorly characterized.
• FBXO21 participates in the ubiquitin-like (Ubl) conjugation pathway, a fundamental mechanism governing protein turnover, signal transduction, and cell cycle control.
• This protein has been identified through high-throughput proteomics studies, confirming its expression in human cells and inclusion in the reference proteome.

Experimental Guidance and Technical Tips

• The immunogen was designed using a recombinant fusion protein spanning amino acids 440–621, a unique region of FBXO21 that may reduce cross-reactivity with closely related F-box proteins; consider this design when interpreting specificity.
• For western blotting, expect a band around 72 kDa based on the predicted molecular weight; however, actual migration may vary with gel conditions and post-translational modifications.
• In immunohistochemistry and immunofluorescence applications, validate staining patterns in known positive and negative tissues, as expression data for FBXO21 are limited.
• This antibody cross-reacts with human, mouse, and rat FBXO21, but always confirm reactivity in your specific model system before large-scale experiments.
• Because FBXO21 is part of the SCF ubiquitin ligase complex, co-immunoprecipitation or proximity-based assays may help interrogate its interaction partners.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a trusted one-stop partner for IVD raw materials, bridging the gap between concept and clinical application. For research into the ubiquitin-proteasome system and targets like FBXO21, we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. Our sourcing network covers rare and specialized targets, supporting seamless assay development from early R&D to final diagnostic deployment.