Anti-FBXO2 Polyclonal Antibody for WB and ELISA - Q9UK22

Core Product Specifications and Parameters

Biological Background: FBXO2 Function and Localization

• FBXO2 (F-box only protein 2), also known as FBG1, FBX2, Fbs1, OCP1, or NFB42, is a member of the F-box protein family and functions as the substrate recognition subunit of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex.
• It specifically recognizes and binds high-mannose-type sugar chains on misfolded glycoproteins that have been retrotranslocated from the endoplasmic reticulum (ER) to the cytosol, targeting them for ubiquitination and proteasomal degradation as part of the ER-associated degradation (ERAD) pathway.
• By promoting the clearance of unfolded glycoproteins, FBXO2 helps prevent the formation of potentially toxic cytosolic protein aggregates, maintaining cellular proteostasis.
• FBXO2 localizes predominantly to the cytoplasm and is associated with microsomal membranes, consistent with its role in ERAD.
• As a lectin-type F-box protein, it is implicated in the ubiquitin-proteasome system and has been identified through proteomics studies. No tissue-specific or antimicrobial functions are reported.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein covering the full-length human FBXO2 (amino acids 1–296), which is expected to generate polyclonal antibodies recognizing multiple epitopes across the protein.
• Given the high sequence homology among mammalian FBXO2 orthologs, the antibody has demonstrated cross-reactivity with mouse and rat samples; consider validating in your specific experimental system.
• The antibody is suitable for western blot (WB) and ELISA; for optimal results, titrate the antibody concentration and use appropriate positive controls expressing FBXO2.
• For WB, note that the calculated molecular weight of FBXO2 is approximately 33 kDa; confirm band specificity using knockout or knockdown controls when possible.

CamelBio: Your One-Stop Sourcing Bridge

We offer a comprehensive suite of IVD raw materials and technical support to streamline your development pipeline from concept to clinic. This anti-FBXO2 polyclonal antibody can be integrated into assays focusing on ubiquitin-mediated proteolysis or glycoprotein quality control pathways. Beyond this product, CamelBio provides validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets to meet your diagnostic and research needs.