Anti-FBXO10 Rabbit Polyclonal Antibody for WB and ELISA - Q9UK96

Core Product Specifications and Parameters

Biological Background: FBXO10 Function and Localization

• FBXO10 is a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.
• Mediates the ubiquitination and subsequent lysosomal degradation of the receptor for advanced glycation end products (RAGE). Related references: PMID:28515150
• Directly controls the ubiquitination and degradation of HGAL (GCSAM), thereby decreasing B-cell receptor (BCR) signaling. Related references: PMID:31570756
• Plays a role in apoptosis by mediating the ubiquitination and degradation of BCL2, an anti-apoptotic protein.
• Localizes predominantly to the cytoplasm.
• Contains an F-box domain, characteristic of this protein family.
• Is a phosphoprotein and undergoes alternative splicing, as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-180) of human FBXO10, which may contribute to specificity. Consider validating in your relevant sample system.
• For Western blot, the predicted molecular weight is approximately 105 kDa. Include appropriate molecular weight markers to confirm band size.
• Cross-reactivity with rat has been observed, but reactivity with other species should be tested independently.
• This antibody is suitable for ELISA applications; optimal working dilutions should be determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. For research on ubiquitination and apoptosis involving FBXO10, we offer validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. Our platform also supports sourcing of rare-target raw materials to accelerate your projects from concept to clinic.