Anti-FBXL5 Polyclonal Antibody for WB, ELISA - Q9UKA1

Core Product Specifications and Parameters

Biological Background: FBXL5 Function and Localization

• FBXL5 (F-box/LRR-repeat protein 5) is a component of the SCF (SKP1-cullin-F-box) E3 ubiquitin-protein ligase complex. It regulates iron homeostasis by targeting IREB2/IRP2 for ubiquitination and degradation. Related references: PMID:19762596, PMID:19762597
• The N-terminal hemerythrin-like (Hr) domain contains a diiron metal center that acts as an iron sensor. Under low iron, FBXL5 is unstable; iron binding stabilizes the protein. Related references: PMID:19762596, PMID:19762597
• The C-terminal domain contains a redox-sensitive [2Fe-2S] cluster, which upon oxidation enables FBXL5 to recruit IRP2 for degradation in an oxygen-dependent manner. Related references: PMID:32126207
• FBXL5 also ubiquitinates the dynactin complex component DCTN1, promoting its degradation. Related references: PMID:17532294
• In the nucleus, FBXL5 ubiquitinates the transcription factor SNAI1, preventing its DNA binding and promoting degradation. Related references: PMID:24157836
• FBXL5 negatively regulates the DNA damage response by mediating degradation of the DNA repair protein NABP2. Related references: PMID:25249620
• It localizes primarily to the cytoplasm and perinuclear region, but also shuttles to the nucleus. Its domains include leucine-rich repeats and iron-sulfur clusters.
• Alternative splicing produces multiple isoforms, as indicated by proteomics identification and reference proteome data.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1–310, corresponding to the N-terminal hemerythrin-like and F-box domains, which are evolutionarily conserved across human, mouse, and rat. This region likely accounts for the observed cross-reactivity.
• For western blotting (WB), consider the theoretical molecular weight of approximately 79 kDa (UniProt) or the supplier-reported 79 kDa. Confirm band size in your specific sample matrix and validate signal specificity using appropriate controls (e.g., knockdown or knockout lysates).
• In ELISA applications, the polyclonal nature may allow detection of both native and denatured target protein. Optimize coating and detection conditions empirically.
• Note that FBXL5 undergoes alternative splicing; therefore, multiple bands may be detected in WB. Validate the antibody’s reactivity profile in the relevant tissue or cell line.
• As with any polyclonal antibody, lot-to-lot consistency should be verified, and we recommend pre-adsorption testing if high specificity is required for your assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For FBXL5 research focusing on ubiquitination, iron metabolism, or DNA repair pathways, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing to accelerate your diagnostic development.