Anti-FBXL2 Rabbit Polyclonal Antibody for WB and ELISA - Q9UKC9

Core Product Specifications and Parameters

Biological Background: FBXL2 Function and Localization

• FBXL2 (F-box/LRR-repeat protein 2), also known as FBL2 or FBL3, is a substrate recognition component of the SCF (SKP1-cullin-F-box protein) E3 ubiquitin-protein ligase complex.
• It mediates ubiquitination and degradation of cyclins CCND2 and CCND3 in a calcium/calmodulin-dependent manner, promoting cell-cycle arrest in G0. Related references: PMID:22020328 PMID:22323446
• SCF(FBXL2) targets PIK3R2 for degradation, thereby modulating PI3K signaling and autophagy (PubMed:23604317). Related references: PMID:23604317
• It negatively regulates inflammation by ubiquitinating TRAF proteins (TRAF1-6) and the NLRP3 inflammasome (PubMed:26037928). Related references: PMID:26037928
• FBXL2 is geranylgeranylated by the GGTase-3 complex, which localizes it to cellular membranes where it degrades membrane-anchored targets (PubMed:31209342, PubMed:32128853). Related references: PMID:31209342 PMID:32128853
• It is expressed in multiple tissues including brain, heart, kidney, liver, lung, pancreas, and placenta.
• Associated keywords: alternative splicing, calmodulin-binding, leucine-rich repeat, lipoprotein, prenylation, phosphoprotein, and Ubl conjugation pathway.

Experimental Guidance and Technical Tips

• The immunogen encompasses residues 120–240 of human FBXL2, a region likely conserved in mouse and rat, supporting observed cross-reactivity. For Western blot, consider using whole-cell lysates from relevant cell lines or tissue extracts; the expected band size is approximately 47 kDa.
• For ELISA applications, validate antibody specificity with recombinant FBXL2 protein or peptide controls, and titrate appropriately for your assay system.
• Note that FBXL2 is membrane-associated; consider appropriate lysis conditions to ensure extraction of membrane proteins.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, offering this FBXL2 antibody as part of a comprehensive portfolio for the ubiquitin-proteasome system, cell cycle, and inflammation research. Beyond individual antibodies, we supply validated antibody pairs, bulk monoclonal/polyclonal reagents, and ancillary reagents, along with technical consulting to support every stage from concept to clinical application.