Anti-Fbx32/FBXO32 Rabbit Monoclonal Antibody for WB, ELISA - Q969P5

Core Product Specifications and Parameters

Biological Background: Fbx32/FBXO32 Function and Localization

• Fbx32/FBXO32 is a member of the F-box protein family, serving as a substrate recognition component of the SCF E3 ubiquitin ligase complex, which targets proteins for ubiquitin-mediated proteasomal degradation.
• It plays a critical role in skeletal muscle atrophy, likely by recognizing and ubiquitinating phosphorylated target proteins.
• Known substrates include TERF1, KLF4 (regulating macrophage efferocytosis), and ANXA1 (mediating LPS-induced apoptosis and mitochondrial dysfunction).
• Mediates cyclin D1 stabilization via 'Lys-27'-linked ubiquitination. Related references: PMID:40307251
• Subcellularly, the protein localizes to both the cytoplasm and nucleus.
• It is specifically expressed in cardiac and skeletal muscle tissue.
• The gene undergoes alternative splicing, and the protein is associated with the ubiquitin conjugation pathway and disease variants.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1-120 of human Fbx32/FBXO32, a region that may be conserved among species; observed cross-reactivity with mouse and rat supports this.
• For Western blot, consider testing dilutions from 1:500 to 1:2000, and validate using lysates from cardiac or skeletal muscle tissues where expression is specific.
• The predicted molecular weight is 42 kDa; ensure appropriate SDS-PAGE gel percentage and transfer conditions for optimal detection.
• For ELISA, the antibody may be used in sandwich or indirect formats; consider pairing with a suitable detection antibody and validate with recombinant protein standards.
• Given its role in ubiquitination and muscle atrophy, using proteasome inhibitors or atrophy models could enhance target detection in experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted partner for IVD raw material sourcing, offering a wide selection of antibodies, antigens, and auxiliary reagents to accelerate your research and diagnostic product development. For targets involved in the ubiquitin-proteasome pathway and muscle atrophy, such as Fbx32/FBXO32, we provide monoclonal antibodies, matched pairs, and bulk supplies to support your assay needs. Our one-stop platform also includes technical services and consulting to help you navigate from early-stage research to clinical application.