Anti-Fbx32/FBXO32 Polyclonal Antibody for WB, IF-P, ELISA - Q969P5

Core Product Specifications and Parameters

Biological Background: Fbx32/FBXO32 Function and Localization

• F-box only protein 32 (FBXO32), also known as Atrogin-1 or muscle atrophy F-box protein (MAFbx), is encoded by the FBXO32 gene and plays a pivotal role in ubiquitin-mediated proteolysis.
• As a substrate recognition component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex, FBXO32 mediates the ubiquitination and subsequent proteasomal degradation of target proteins, particularly during skeletal muscle atrophy.
• FBXO32 specifically recognizes and binds to phosphorylated target proteins, facilitating their degradation and thereby regulating muscle mass.
• Subcellularly, FBXO32 localizes to both the cytoplasm and nucleus, consistent with its role in protein turnover across cellular compartments.
• FBXO32 is specifically expressed in cardiac and skeletal muscle, underscoring its tissue-specific function in muscle biology.
• Beyond muscle atrophy, FBXO32 negatively regulates macrophage efferocytosis by promoting ubiquitination of KLF4, and regulates LPS-induced apoptosis and mitochondrial dysfunction through ANXA1 ubiquitination (By similarity).
• Notably, FBXO32 mediates stabilization of cyclin D1 protein via 'Lys-27'-linked ubiquitination (PubMed:40307251). Related references: PMID:40307251

Experimental Guidance and Technical Tips

• For Western blot (WB), consider using lysates from skeletal muscle or cardiac tissue, where FBXO32 is highly expressed. Validate detection at the expected molecular weight of ~42 kDa.
• For immunofluorescence (IF-P), optimize fixation and permeabilization conditions to visualize nuclear and cytoplasmic localization patterns in muscle cells.
• For ELISA, use the recombinant immunogen (amino acids 206-355) as a positive control and include appropriate negative controls to ensure specificity.
• As a polyclonal antibody, lot-to-lot consistency should be verified, and it is recommended to titrate the antibody for each application and sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable access to high-quality IVD raw materials, including validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets. This anti-FBXO32 polyclonal antibody supports research into muscle atrophy, ubiquitin-proteasome pathways, and related disease mechanisms. With a comprehensive supply network and technical consultation, CamelBio streamlines your development pipeline from concept to clinic.