Anti-Fbx32/FBXO32 Polyclonal Antibody for WB, IF-P, ELISA - Q969P5

Core Product Specifications and Parameters

Biological Background: Fbx32/FBXO32 Function and Localization

• FBXO32, also known as Atrogin-1 or MAFbx, is an F-box only protein 32 that acts as a substrate recognition component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex.
• It mediates ubiquitination and subsequent proteasomal degradation of target proteins, with a key role in skeletal muscle atrophy.
• Among its identified substrates are TERF1, the transcription factor KLF4 (suppressing MERTK transcription to negatively regulate macrophage efferocytosis), and ANXA1 (promoting LPS-induced apoptosis and mitochondrial dysfunction); these interactions have been reported by similarity.
• FBXO32 mediates cyclin D1 protein stabilization via 'Lys-27'-linked ubiquitination. Related references: PMID:40307251
• The protein localizes to both the cytoplasm and nucleus.
• It is specifically expressed in cardiac and skeletal muscle tissue.
• Disease relevance is indicated by database associations between FBXO32 and human disease variants, and it is classified under the ubiquitin conjugation pathway.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant fragment spanning amino acids 206–355 of human FBXO32; this region lies in the C-terminal portion of the protein, which may influence epitope accessibility depending on the protein's conformation or interaction partners.
• For Western blot, a specific band near 42 kDa is anticipated; however, migration may be affected by post-translational modifications or protein degradation, so validate with appropriate positive controls (e.g., lysates from skeletal muscle or cardiac tissue) and molecular weight markers.
• In immunofluorescence (IF-P), expect both nuclear and cytoplasmic staining based on reported subcellular distribution; include appropriate fixation and permeabilization protocols to detect both pools.
• For ELISA, consider using the antibody as either capture or detection reagent with a standard buffer system, and optimize the antibody concentration and incubation conditions for your specific assay format.
• Cross-reactivity with mouse FBXO32 has been confirmed, making it suitable for studies in mouse models of muscle atrophy; however, always verify species-specific signals in your own experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to streamline your assay development. For researchers focused on ubiquitin-proteasome biology and muscle atrophy, we provide reliable access to anti-FBXO32 antibodies and can source rare-target reagents tailored to your project needs. From concept to clinic, CamelBio bridges your procurement gaps with expert technical consulting and a comprehensive supply chain.