Anti-FBP2 Rabbit Polyclonal Antibody for WB, IF-P, ELISA - O00757

Core Product Specifications and Parameters

Biological Background: FBP2 Function and Localization

• FBP2 encodes fructose-1,6-bisphosphatase isozyme 2, also known as muscle FBPase, a key enzyme in gluconeogenesis.
• It catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate in the presence of divalent cations, contributing to glycogen synthesis from lactate and other carbohydrate precursors.
• FBP2 belongs to the fructose-1,6-bisphosphatase family and is one of two isozymes; the other is the liver isozyme (FBP1).
• The enzyme is localized in the cytoplasm and nucleus, and additionally at cell junctions and the Z lines of sarcomeres in muscle cells.
• Tissue specificity: FBP2 protein is predominantly expressed in skeletal muscle.
• FBP2 is an allosteric enzyme regulated by metal ions (Ca2+, Mg2+) and is subject to phosphorylation; structural studies are available.
• Mutations in FBP2 are associated with disease variants, including leukodystrophy, underscoring its physiological importance.

Experimental Guidance and Technical Tips

• For Western blotting (WB), a band around 37 kDa is predicted; consider using human skeletal muscle lysate as a positive control.
• In immunofluorescence paraffin (IF-P) assays, expect localization to cytoplasm and muscle sarcomeres; validate expression patterns in relevant tissue sections.
• Cross-reactivity with mouse and rat extends utility to rodent models; confirm reactivity in your specific sample system.
• For ELISA applications, coating with recombinant FBP2 protein may optimize detection; titrate antibody concentration for best signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a streamlined sourcing experience for IVD raw materials, including validated antibodies against metabolic targets like FBP2. Our portfolio features optimized polyclonal and monoclonal antibodies, complemented by bulk ancillary reagents and custom sourcing for rare targets, empowering your assay development from concept to clinic.