Anti-FBP2 Polyclonal Antibody for WB, ELISA - O00757

Core Product Specifications and Parameters

Biological Background: FBP2 Function and Localization

• FBP2 (Fructose-1,6-bisphosphatase isozyme 2), also known as Muscle FBPase, is a key enzyme in gluconeogenesis and glycogen synthesis, catalyzing the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate in the presence of divalent cations.
• It predominantly participates in glycogen synthesis from carbohydrate precursors like lactate, linking glycolysis to anabolic pathways in muscle.
• Encoded by the FBP2 gene, it belongs to the fructose-1,6-bisphosphatase family and shares structural features with the liver isozyme FBP1, though it exhibits distinct regulatory properties and tissue expression.
• Subcellularly, FBP2 localizes to the cytoplasm, nucleus, and cell junctions, with specific association with myofibril sarcomeres at the Z-line in skeletal muscle.
• Its activity is regulated allosterically by calcium and magnesium ions and likely by post-translational modifications such as phosphorylation, as indicated by proteomics.
• Protein expression is highly tissue-specific, detected primarily in skeletal muscle, consistent with its role in muscle metabolism.
• Keywords and disease links associate FBP2 with carbohydrate metabolism, allosteric enzyme regulation, and leukodystrophy, highlighting its importance in both normal physiology and disease.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein spanning amino acids 1–339 of human FBP2, covering the full-length sequence. This may generate antibodies recognizing both native and denatured protein forms.
• For Western blot, consider using reducing conditions and a positive control lysate from human, mouse, or rat skeletal muscle to confirm a band at ~37 kDa.
• When using ELISA, titration of both the capture and detection antibodies is recommended to achieve optimal signal-to-noise ratios.
• Cross-reactivity with mouse and rat orthologs is indicated; however, validation in your specific sample matrix is advised to ensure consistency.
• Given the enzyme's involvement in gluconeogenesis, appropriate metabolic pathway inhibitors or activators may be used to assess functional relevance in cell-based assays.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we understand the critical need for reliable IVD raw materials, and our FBP2 Rabbit pAb is just one example of our commitment to supporting diagnostic manufacturers and researchers. We provide a comprehensive portfolio of antibodies, including validated pairs, optimized monoclonals and polyclonals, and bulk ancillary reagents to streamline your gluconeogenesis and metabolic disease research. Whether you need standard catalog products or rare-target sourcing, our one-stop platform bridges you from concept to clinic, simplifying your supply chain.