Anti-FBP1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P09467

Core Product Specifications and Parameters

Biological Background: FBP1 Function and Localization

• Fructose-1,6-bisphosphatase 1 (FBP1) is a rate-limiting enzyme in gluconeogenesis, catalyzing the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate in the presence of divalent cations.
• FBP1 plays a critical role in regulating glucose sensing and insulin secretion in pancreatic beta-cells.
• In the liver, FBP1 modulates glycerol gluconeogenesis, contributing to metabolic homeostasis.
• Beyond its enzymatic function, FBP1 acts as an important regulator of appetite and adiposity; increased hepatic expression after nutrient excess elevates circulating satiety hormones and suppresses appetite-stimulating neuropeptides, providing feedback to limit weight gain.
• FBP1 is expressed in pancreatic islets, consistent with its role in insulin secretion and glucose metabolism.
• The enzyme requires magnesium or other divalent metals for activity and is subject to allosteric regulation, as well as post-translational modifications including phosphorylation and acetylation.
• Mutations in FBP1 are associated with metabolic disorders, highlighting its clinical relevance.

Experimental Guidance and Technical Tips

• For Western blotting, expect a band at approximately 37 kDa; we recommend titrating the antibody to determine optimal dilution and including appropriate positive and negative controls.
• In immunofluorescence/ICC, ensure adequate fixation and permeabilization; validate staining specificity using FBP1-expressing cell lines or tissue sections.
• The antibody's cross-reactivity with mouse and rat enables translational studies across these species; however, confirm performance in your specific experimental settings.
• As a rabbit polyclonal antibody, lot-to-lot consistency may vary; consider including internal reference samples for comparative analysis between experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials, serving as your one-stop partner from concept to clinic. This anti-FBP1 polyclonal antibody supports metabolism and gluconeogenesis research; beyond this product, CamelBio can source validated antibody pairs, bulk ancillary reagents, and rare-target raw materials to accelerate your diagnostic development.