Anti-FBP1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P09467

Core Product Specifications and Parameters

Biological Background: FBP1 Function and Localization

• Fructose-1,6-bisphosphatase 1 (FBP1), also known as liver FBPase, is a key gluconeogenic enzyme that catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate, a rate-limiting step in glucose production.
• FBP1 plays a crucial role in glucose sensing and insulin secretion of pancreatic beta-cells, linking gluconeogenesis to endocrine function.
• In the liver, FBP1 modulates glycerol gluconeogenesis, influencing hepatic glucose output.
• FBP1 acts as a regulator of appetite and adiposity; increased hepatic expression after nutrient excess elevates circulating satiety hormones and reduces appetite-stimulating neuropeptides, providing a feedback mechanism to limit weight gain.
• FBP1 is primarily known as the liver isoform; its expression has been specifically noted in pancreatic islets.
• The enzyme is subject to allosteric regulation, acetylation, and phosphorylation, and requires divalent metal ions (magnesium) for activity.
• Genetic variants in FBP1 are linked to metabolic disorders (keyword: Disease variant), underscoring its clinical relevance.

Experimental Guidance and Technical Tips

• This monoclonal antibody was raised against a synthetic peptide spanning amino acids 1–100 of human FBP1, targeting the N-terminal region; it is predicted to recognize the full-length protein.
• For Western blot (WB), a band migrating at approximately 37 kDa is expected in lysates from human, mouse, or rat tissues. Verify specificity using appropriate positive and negative controls (e.g., FBP1-expressing vs. knockdown cells).
• For immunohistochemistry (IHC-P), consider testing on pancreatic or liver tissue sections; validate staining specificity by comparing with isotype control or peptide blocking.
• For ELISA, the antibody can be used as a capture or detection reagent; optimize coating concentrations and detection systems for your specific assay format.
• As with any reagent, performance should be validated in the relevant sample system and under your experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This high-quality anti-FBP1 antibody supports research in glucose metabolism, diabetes, and obesity, and can be seamlessly integrated into your assay development pipeline. Beyond this product, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and sourcing for rare-target raw materials to power your diagnostic innovations.