Anti-FBP1 Rabbit Monoclonal Antibody for WB, IF/ICC, IF-P, ELISA - P09467

Core Product Specifications and Parameters

Biological Background: Fructose-1,6-bisphosphatase 1 Function and Localization

• FBP1 encodes fructose-1,6-bisphosphatase 1 (also known as Liver FBPase or D-fructose-1,6-bisphosphate 1-phosphohydrolase 1), a key regulatory enzyme in gluconeogenesis.
• Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate in the presence of divalent cations such as Mg²⁺, serving as a rate-limiting step in glucose production.
• Plays a role in glucose sensing and insulin secretion of pancreatic beta-cells; expressed in pancreatic islets.
• Modulates glycerol gluconeogenesis in the liver, contributing to metabolic homeostasis.
• Functions as an important regulator of appetite and adiposity; increased expression in the liver after nutrient excess elevates circulating satiety hormones and reduces appetite-stimulating neuropeptides, providing a feedback mechanism to limit weight gain.
• Subject to post-translational modifications including acetylation and phosphorylation; classified as an allosteric enzyme, metal-binding, and a phosphoprotein.
• Associated with disease variants; included in proteomics identification and direct protein sequencing studies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 239–338 of human FBP1, a region that may effectively detect the native protein in various assay formats.
• For Western blotting, a band at approximately 37 kDa (calculated MW: 36.8 kDa) is expected; use appropriate controls.
• IF/ICC and IF-P applications require optimized fixation and permeabilization protocols; validate in the relevant cell or tissue samples.
• Reported cross-reactivity with mouse and rat suggests suitability for these species, but independent verification is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing solution for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support diagnostic development. This anti-FBP1 rabbit monoclonal antibody is intended for research in glucose metabolism and appetite regulation. For rare or custom targets, CamelBio’s supply network can source specialized raw materials to help move your project from concept to clinic.