Anti-FBLN5 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9UBX5

Core Product Specifications and Parameters

Biological Background: Fibulin-5 Function and Localization

• Fibulin-5 (FBLN5, also known as DANCE or UP50) is a secreted extracellular matrix glycoprotein belonging to the fibulin family, characterized by tandem EGF-like domains and an RGD motif.
• It plays an essential role in elastic fiber formation by promoting the assembly of continuous elastin polymer and facilitating interactions between microfibrils and elastin.
  Related references: PMID:18185537
• Fibulin-5 stabilizes and organizes elastic fibers in tissues such as skin, lung, and vasculature, and may act as an adapter mediating the interaction between fibrillin-1 (FBN1) and elastin (ELN).
  Related references: PMID:17255108
• It promotes adhesion of endothelial cells through interaction with integrin receptors via its RGD motif, functioning as a vascular ligand involved in vascular development and remodeling.
  Related references: PMID:10428823
• The protein is predominantly expressed in heart, ovary, and colon, with additional expression in kidney, pancreas, testis, lung, and placenta, but is absent from brain, liver, thymus, prostate, and peripheral blood leukocytes; at the protein level, it is detected in skin fibroblasts.
  Related references: PMID:10428823, PMID:17035250
• Subcellularly, FBLN5 is secreted and localizes to the extracellular space and extracellular matrix.
• Mutations in FBLN5 are associated with age-related macular degeneration, Charcot-Marie-Tooth disease, and other connective tissue disorders, underscoring its clinical importance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 199-448 of human FBLN5; consider verifying sequence homology if using in other species such as mouse and rat, as cross-reactivity is reported.
• For Western blot, the expected molecular weight is approximately 50 kDa; include appropriate molecular weight markers and optimize gel percentage and transfer conditions.
• In immunofluorescence/ICC, since FBLN5 is a secreted extracellular matrix protein, consider including appropriate permeabilization and fixation steps to detect matrix-associated protein.
• As a polyclonal antibody, lot-to-lot consistency may vary; it is recommended to validate performance in the intended experimental system.
• For ELISA, the recombinant immunogen protein can serve as a positive control to establish assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with a comprehensive one-stop platform for IVD raw material sourcing, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For researchers investigating elastic fiber biology and extracellular matrix dynamics, we offer reliable access to high-quality anti-FBLN5 antibodies and can assist with rare target sourcing and technical consultation. From concept to clinic, CamelBio is your trusted partner in advancing diagnostic development.