Anti-Fatty Acid Synthase (FASN) Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P49327

Core Product Specifications and Parameters

Biological Background: Fatty Acid Synthase (FASN) Function and Localization

• Fatty acid synthase (FASN) is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids from acetyl-CoA and malonyl-CoA using NADPH.
• The enzyme contains seven distinct catalytic activities and an acyl carrier protein (ACP) domain that binds the prosthetic group 4'-phosphopantetheine.
• (Microbial infection) FASN activity is required for the replication of SARS-CoV-2, highlighting its role in viral infection.
• Subcellular localization includes cytoplasm and melanosome.
• Ubiquitously expressed, with prominent levels in brain, lung, liver, and mammary gland.
• Post-translational modifications include acetylation, phosphorylation, S-nitrosylation, and ubiquitination, as inferred from UniProt keywords.
• The protein has a predicted molecular weight of approximately 273 kDa, consistent with its large multidomain architecture.
• Classified as a hydrolase, lyase, oxidoreductase, and transferase, underscoring its catalytic diversity in lipid metabolism.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the C-terminal region (aa 2400-2500) of human FASN; consider verifying epitope conservation in mouse and rat if cross-species reactivity is critical.
• For western blotting, due to the large protein size (~273 kDa), use low-percentage SDS-PAGE gels and optimize transfer conditions to ensure efficient blotting.
• In immunohistochemistry (IHC) and immunofluorescence (IF), validate the antibody in your tissue of interest with appropriate antigen retrieval and blocking protocols.
• For immunoprecipitation (IP) and ELISA, confirm compatibility under non-denaturing conditions if native conformation is required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting, covering every stage from concept to clinic. For fatty acid metabolism and related research, we offer validated antibody pairs, optimized recombinant monoclonal antibodies, and bulk ancillary reagents to support your assays. Whether you need routine or rare target raw materials, CamelBio ensures reliable supply and dedicated technical support to accelerate your development.