Anti-FATP4/SLC27A4 Monoclonal Antibody for WB, ELISA - Q6P1M0

Core Product Specifications and Parameters

Biological Background: FATP4/SLC27A4 Function and Localization

• FATP4 (SLC27A4) is a long-chain fatty acid transport protein and acyl-CoA ligase, mediating fatty acid transport across the plasma membrane and catalyzing ATP-dependent formation of fatty acyl-CoA (PubMed:10518211, PubMed:12556534, PubMed:20448275, PubMed:22022213, PubMed:24269233). Related references: PMID:10518211, PMID:12556534, PMID:20448275, PMID:22022213, PMID:24269233
• Acts as the principal fatty acid transporter in small intestinal enterocytes, essential for fat absorption in early embryogenesis (PubMed:20448275). Related references: PMID:20448275
• Functions in the formation of the epidermal barrier and is likely involved in fatty acid transport across the blood-brain barrier (PubMed:21395585). Related references: PMID:21395585
• Localizes to the endoplasmic reticulum membrane, consistent with its role in lipid metabolism.
• Belongs to the solute carrier family 27 (SLC27), also known as fatty acid transport proteins (FATPs); alternative splicing produces multiple isoforms.
• Highly expressed in brain, testis, colon, kidney, and adipose tissue; lower expression in heart, liver, small intestine, stomach, and leukocytes.
• Associated with disease variants, including ichthyosis; involved in preventing light-induced degeneration of rods and cones.
• Keywords: Alternative splicing, Disease variant, Endoplasmic reticulum, Fatty acid metabolism, Ligase, Lipid metabolism, Lipid transport, Membrane, Nucleotide-binding, Transmembrane helix, Transport.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a conserved region (amino acids 43-237) of human FATP4, which shares high homology with the mouse ortholog, supporting observed cross-reactivity.
• For Western blotting, a band of approximately 72 kDa is expected; validate detection in human or mouse tissue/cell lysates known to express FATP4 (e.g., intestine, liver, or adipose tissue).
• ELISA performance may be optimized using recombinant or purified FATP4 protein; include appropriate positive and negative controls.
• Consider verifying antibody specificity in knockout or knockdown models, as FATP4 belongs to a protein family with potential cross-reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio delivers a comprehensive IVD raw-material supply solution, from validated antibody pairs and optimized monoclonal antibodies to bulk ancillary reagents for lipid metabolism and fatty acid transport research. Leveraging this anti-FATP4 rabbit mAb as a lead candidate, we support diagnostic manufacturers and laboratories with expert sourcing and technical services to accelerate assay development from concept to clinic.