Anti-FATP2/SLC27A2 Monoclonal Antibody for WB, IHC-P, ELISA - O14975

Core Product Specifications and Parameters

Biological Background: FATP2/SLC27A2 Function and Localization

• FATP2/SLC27A2, also known as Long-chain fatty acid transport protein 2 or Very long-chain acyl-CoA synthetase (VLACS), is encoded by the SLC27A2 gene.
• It mediates the import of long-chain fatty acids (LCFA) into cells by facilitating transport across cell membranes, crucial for hepatic fatty acid uptake. Related references: PMID:10198260 PMID:10749848 PMID:11980911
• Functions as an acyl-CoA ligase, catalyzing ATP-dependent formation of fatty acyl-CoA using LCFA and very-long-chain fatty acids (VLCFA) as substrates, preventing efflux and promoting uptake. Related references: PMID:10198260 PMID:10749848 PMID:11980911
• Plays a pivotal role in regulating LCFA substrates for beta-oxidation or triglyceride synthesis in tissues. Related references: PMID:20530735
• Can activate branched-chain fatty acids like phytanic and pristanic acid, and may contribute to sphingosine-1-phosphate synthesis. Related references: PMID:10198260 PMID:24269233
• Localizes to the endoplasmic reticulum, peroxisome, and cell membranes, consistent with roles in fatty acid transport and metabolism.
• Predominantly expressed in liver, with lower levels in kidney, placenta, intestine, brain, heart, and colon. Related references: PMID:10198260 PMID:21768100 PMID:24269233 PMID:20530735
• Post-translationally modified by acetylation and phosphorylation; alternative splicing produces multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen consists of a recombinant fragment (amino acids 494-620) from the C-terminal region of human FATP2, ensuring specificity for this target.
• For Western blot (WB), use whole cell or tissue lysates from human, mouse, or rat samples expressing FATP2; the predicted molecular weight is ~70 kDa.
• For immunohistochemistry (IHC-P), consider testing on formalin-fixed, paraffin-embedded liver or kidney sections where expression is robust; optimize antigen retrieval and antibody dilution.
• For ELISA, the antibody can be employed as a capture or detection reagent; assay conditions should be empirically determined.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your reliable one-stop partner for IVD raw materials, offering validated antibodies, bulk reagents, and customized sourcing solutions. Our FATP2/SLC27A2 monoclonal antibody supports research in fatty acid transport and lipid metabolism, complementing our portfolio of cell signaling and metabolic pathway products. Whether you need matched antibody pairs, optimized monoclonals, or rare target sourcing, CamelBio streamlines your path from concept to clinic.