Anti-FAT10/UBD Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O15205

Core Product Specifications and Parameters

Biological Background: FAT10/UBD Function and Localization

• FAT10 (Ubiquitin D) is a ubiquitin-like protein modifier that can be covalently conjugated to target proteins, targeting them for degradation by the 26S proteasome in a NUB1-dependent manner. Related references: PMID:15831455, PMID:16707496, PMID:19166848
• Conjugation is activated by the E1-like enzyme UBA6 through adenylation of the C-terminal glycine. Related references: PMID:17889673, PMID:35970836
• FAT10 promotes the expression of the immunoproteasome subunit PSMB9/LMP2 and regulates NF-κB activation by mediating TNF-induced proteasomal degradation of ubiquitinated IκBα. Related references: PMID:19959714
• Involved in dendritic cell maturation and constitutively expressed in mature dendritic cells and B cells, as well as in tissues of the reticuloendothelial system, gastrointestinal system, kidney, lung, and prostate. Related references: PMID:19028597
• Mediates mitotic non-disjunction and chromosome instability by impairing the kinetochore localization of MAD2L1 during prometaphase. Related references: PMID:16495226
• Under conditions of proteasome saturation or impairment, FAT10 is involved in the formation of aggresomes. Related references: PMID:19033385
• FAT10 can induce caspase-dependent apoptosis, particularly in renal epithelium and tubular cells, contributing to kidney diseases like polycystic kidney disease and HIV-associated nephropathy. Related references: PMID:16495380
• Subcellularly localized to both the nucleus and cytoplasm, FAT10 undergoes acetylation and participates in the ubiquitin-like conjugation pathway.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein corresponding to amino acids 1-165 of human FAT10/UBD. Consider this when designing epitope mapping or antibody characterization experiments.
• For Western blotting, the predicted protein weight is 18 kDa; use appropriate molecular weight markers and loading controls in this range.
• In immunofluorescence/immunocytochemistry (IF/ICC), optimize fixation/permeabilization conditions to preserve the nuclear and cytoplasmic localization of FAT10/UBD.
• This antibody shows cross-reactivity with mouse FAT10/UBD, making it suitable for studies in mouse models, but in-species validation is recommended.
• For ELISA applications, perform standard checkerboard titrations to determine optimal coating antibody and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive one-stop access to IVD raw materials, technical services, and consulting for diagnostic manufacturers, laboratories, and research institutes—from concept to clinic. Our FAT10/UBD rabbit polyclonal antibody is an excellent choice for studies in the ubiquitin-proteasome system, immune regulation, and renal pathology. Beyond this product, CamelBio can source validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw materials to support your entire development pipeline.