Anti-FAT10/UBD Polyclonal Antibody for IF/ICC, ELISA - O15205

Core Product Specifications and Parameters

Biological Background: FAT10/UBD Function and Localization

• FAT10/UBD (ubiquitin D) is a ubiquitin-like protein modifier consisting of two tandem ubiquitin-like domains, leading to the alternative name “diubiquitin.”
• Conjugated to target proteins via UBA6-mediated adenylation of its C-terminal glycine, it tags them for 26S proteasomal degradation in a NUB1-dependent manner.
  Related references: PMID:15831455, PMID:16707496, PMID:19166848, PMID:17889673, PMID:35970836
• Positively regulates NF-kappa-B signaling by promoting TNF-mediated proteasomal degradation of ubiquitinated I-kappa-B-alpha, and is required for TNF-induced p65 nuclear translocation in renal tubular epithelial cells.
  Related references: PMID:19959714
• Participates in dendritic cell maturation, promoting the differentiation of immature dendritic cells into fully competent antigen-presenting cells that initiate T-cell responses.
  Related references: PMID:19028597
• Mediates chromosome instability and mitotic non-disjunction in long-term cultures and cancers by abbreviating mitosis and impairing MAD2L1 kinetochore localization during prometaphase.
  Related references: PMID:16495226
• Facilitates the formation of aggresomes under conditions of proteasomal saturation or impairment.
  Related references: PMID:19033385
• Triggers caspase-dependent apoptosis, particularly in renal epithelium and tubular cells, and is implicated in kidney pathologies including polycystic kidney disease and HIV-associated nephropathy.
  Related references: PMID:16495380
• Localizes to both the nucleus and cytoplasm, and is constitutively expressed in mature dendritic cells, B-cells, and tissues of the reticuloendothelial system (e.g., thymus, spleen), as well as the gastrointestinal tract, kidney, lung, and prostate.

Experimental Guidance and Technical Tips

• The immunogen (aa 1-165) spans the N-terminal ubiquitin-like domain; sequence alignment with mouse and rat orthologs may inform cross-reactivity expectations.
• For IF/ICC, consider using cell lines known to express FAT10, such as TNF-treated HeLa or primary dendritic cells, and include appropriate controls.
• In ELISA applications, optimize antibody coating and detection conditions, noting the small molecular weight (18 kDa) of the target protein.
• FAT10 expression can be upregulated by inflammatory stimuli (e.g., TNF, LPS) or proteasome inhibition; pre-treat cells accordingly to enhance detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. With expertise in ubiquitin-like modification pathways and innate immunity targets such as FAT10/UBD, we provide one-stop access to rare-target raw materials, technical services, and consulting to accelerate your diagnostic development from concept to clinic.