Anti-FAT10/UBD Monoclonal Antibody for WB and ELISA - O15205

Core Product Specifications and Parameters

Biological Background: FAT10/UBD Function and Localization

• FAT10/UBD (Ubiquitin D), also known as diubiquitin, is a ubiquitin-like protein that functions as a covalent modifier targeting substrates for 26S proteasomal degradation in an NUB1-dependent manner. Conjugation is mediated by the E1 enzyme UBA6. Related references: PMID:15831455 PMID:16707496 PMID:19166848 PMID:17889673 PMID:35970836
• It positively regulates NF-κB signaling by promoting the TNF-induced proteasomal degradation of ubiquitinated IκBα. Related references: PMID:19959714
• FAT10 contributes to chromosome instability by abbreviating mitosis and impairing the kinetochore localization of MAD2L1 during prometaphase. Related references: PMID:16495226
• It promotes caspase-dependent apoptosis, particularly in renal epithelial cells, and is implicated in polycystic kidney disease and HIV-associated nephropathy. Related references: PMID:16495380
• FAT10 is involved in dendritic cell maturation, facilitating the transition of immature DCs into fully competent antigen-presenting cells. Related references: PMID:19028597
• Under proteotoxic stress, FAT10 may facilitate aggresome formation when proteasome capacity is exceeded. Related references: PMID:19033385
• It upregulates the expression of the immunoproteasome subunit PSMB9/LMP2.
• Subcellularly, FAT10 localizes to both the nucleus and cytoplasm.
• It is constitutively expressed in mature dendritic cells and B-cells, with highest levels in lymphoid tissues, gastrointestinal tract, kidney, lung, and prostate.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide covering amino acids 70–150 of human FAT10/UBD. Consider using this region-specific design to minimize cross-reactivity with other ubiquitin-like proteins.
• For Western blot, test on whole-cell lysates from cell lines known to express FAT10, such as human B-cell or dendritic cell lines, or upon TNF/LPS stimulation to induce endogenous expression. Mouse cell lines may also be used given confirmed cross-reactivity.
• In ELISA, the antibody may serve as a capture or detection reagent for quantifying FAT10 levels. Validate assay conditions with recombinant FAT10 protein or cell extracts.
• Because FAT10 can form conjugates and may appear at higher molecular weights, consider running samples with reducing agents and monitoring for ubiquitinated species in WB.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw material supplier, offering one-stop access to validated antibodies, antigen pairs, and auxiliary reagents for diagnostic and research applications. This anti-FAT10/UBD monoclonal antibody supports studies on ubiquitin-like protein modification, inflammation, and cancer biology. For scientists developing assays targeting FAT10, CamelBio can assist with bulk ordering, custom antibody services, and complementary raw materials to accelerate your project from concept to clinic.