Anti-Fascin/FSCN1 Monoclonal Antibody for WB, IHC-P, ELISA - Q16658

Core Product Specifications and Parameters

Biological Background: Fascin Function and Localization

• Fascin (FSCN1) is a 55 kDa actin-bundling protein containing two major actin-binding sites that organize filamentous actin into parallel bundles, essential for the formation of microspikes, membrane ruffles, and stress fibers. Related references: PMID:21685497 PMID:23184945
• It plays a critical role in generating diverse cell protrusions, including filopodia, invadopodia, and microvilli, thereby promoting cell motility and migration. Related references: PMID:20393565 PMID:21685497 PMID:23184945
• Fascin mediates nerve growth factor (NGF)-induced reorganization of the actin cytoskeleton and axon growth cone collapse. Related references: PMID:22155786
• The protein localizes to the cytoplasm, cell cortex, stress fibers, and various cell projections including filopodia and invadopodia, and is found at cell junctions.
• FSCN1 is ubiquitously expressed in human tissues and is subject to post-translational modifications including acetylation, phosphorylation, and ubiquitination, which regulate its activity and stability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 249-362 of human Fascin, a region within the C-terminal actin-binding domain. This region may be conserved across species, supporting the observed cross-reactivity with mouse and rat.
• For Western blotting, the expected molecular weight is approximately 55 kDa. Consider using whole-cell lysates from HeLa, Jurkat, or A431 cells as positive controls.
• For immunohistochemistry (IHC-P), antigen retrieval conditions (e.g., citrate buffer pH 6.0) and optimal antibody dilution should be empirically determined. Validate staining in relevant tissue sections known to express fascin, such as colon or breast epithelium.
• The antibody is also suitable for ELISA; ensure appropriate coating and detection conditions are optimized for the target antigen.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutions with one-stop access to IVD raw materials, technical services, and consulting across the entire development pipeline. This anti-Fascin monoclonal antibody exemplifies our portfolio of validated cytoskeleton research tools suitable for oncology and cell migration studies. Beyond individual antibodies, we supply optimized antibody pairs, bulk ancillary reagents, and customized sourcing for rare target raw materials, ensuring a reliable supply chain from concept to clinic.