Anti-Fas Monoclonal Antibody for WB, IHC-P, ELISA - P25445

Core Product Specifications and Parameters

Biological Background: Fas Function and Localization

• Fas (also known as CD95, APO-1, TNFRSF6) is a member of the tumor necrosis factor receptor superfamily, encoded by the FAS gene on chromosome 10. Multiple isoforms exist due to alternative splicing; secreted isoforms 2–6 can block apoptosis in vitro.
• Acts as a receptor for TNFSF6/FASLG, triggering the formation of the death-inducing signaling complex (DISC) via recruitment of FADD and caspase-8, leading to activation of the caspase cascade and apoptosis.
• Plays a critical role in immune homeostasis, including peripheral tolerance induction and activation-induced cell death of mature T‑cells.
• Subcellular localization includes the cell membrane and membrane rafts; several isoforms are also secreted.
• Post‑translational modifications include disulfide bonds, glycosylation, palmitoylation, and phosphorylation. The protein is also a calmodulin‑binding lipoprotein.
• Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells; upon activation, isoform 1 increases while isoform 6 decreases.
• Genetic variations are associated with autoimmune lymphoproliferative syndrome (ALPS) and other disease variants.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 50–150 of human Fas (NP_000034.1). This region may target extracellular domains; verify epitope accessibility in native conformations.
• Recommended applications (WB, IHC-P, ELISA) are based on supplier data. Optimize dilutions and conditions for your specific assay.
• Detects a protein weight range of 9–38 kDa, likely reflecting various isoforms and/or post‑translational modifications. Use appropriate molecular weight markers.
• Cross‑reactivity is listed for human and rat; validate in the relevant sample system if using other species.
• For immunohistochemistry, consider antigen retrieval protocols due to transmembrane domain epitope accessibility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials for apoptosis research, including validated anti‑Fas monoclonal antibodies, optimized antibody pairs, and ancillary reagents. As a dedicated B2B partner, we streamline procurement for diagnostic manufacturers and labs, ensuring reliable access to rare‑target antibodies and bulk‑quantity ancillary materials. Let CamelBio be your bridge from concept to clinic, with one‑stop technical and sourcing support for all your assay development needs.