Anti-FARSB Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9NSD9

Core Product Specifications and Parameters

Biological Background: FARSB Function and Localization

• Encodes the beta subunit of phenylalanyl-tRNA synthetase (PheRS), a heterotetrameric enzyme that catalyzes the attachment of phenylalanine to its cognate tRNA in the cytoplasm.
• Forms a functional complex with the alpha subunit (FARSA) to charge tRNA(Phe) with phenylalanine, an essential step in protein biosynthesis.
• Localizes predominantly to the cytoplasm, consistent with its role in cytosolic translation.
• The protein contains ATP-binding and metal-binding sites, typical of class II aminoacyl-tRNA synthetases, and its 3D structure has been resolved.
• Undergoes alternative splicing, generating multiple transcript variants that may contribute to functional diversity.
• Genetic variations in FARSB are associated with human diseases, including intellectual disability and neurodegeneration, highlighting its clinical relevance.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 240–589, covering a central/functional region of FARSB. For Western blot, a band around 66 kDa is expected; consider using whole-cell lysates from HeLa or HEK293 as positive controls.
• In immunofluorescence/ICC, anticipate cytoplasmic staining. Co-stain with organelle-specific markers to confirm localization and minimize background.
• Cross-reactivity is reported for human, mouse, and rat. When using samples from these species, validate specificity under your experimental conditions.
• For ELISA, the polyclonal nature may enhance detection sensitivity; titrate the antibody and use appropriate blocking to reduce nonspecific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with a complete range of IVD raw materials for protein synthesis and aminoacylation studies. Beyond this anti-FARSB antibody, we supply validated antibody pairs, optimized recombinant proteins, and bulk ancillary reagents. Contact us for custom sourcing of rare-target raw materials and technical guidance to accelerate your project from concept to clinic.